DETECTION OF A TRANSFORMATION-RELATED ANTIGEN IN CHEMICALLY-INDUCED SARCOMAS AND OTHER TRANSFORMED-CELLS OF THE MOUSE
DETECTION OF A TRANSFORMATION-RELATED ANTIGEN IN CHEMICALLY-INDUCED SARCOMAS AND OTHER TRANSFORMED-CELLS OF THE MOUSE
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DOI：
10.1073/pnas.76.5.2420
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
OLD, LJ
中科院分区：
文献类型：
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作者：
DELEO, AB;JAY, G;OLD, LJ
抗BALB/c甲硫氨酸肉瘤制备的抗血清在同系或兼容的F1小鼠识别的蛋白质与[35 S]蛋氨酸标记的转化BALB/c细胞的提取物中的表观分子量为53，000。该组分，命名为p53，在正常成年小鼠成纤维细胞、淋巴细胞或造血细胞或小鼠胚胎细胞或3 T3 [成纤维细胞]细胞中未检测到。广泛的各种抗血清，包括针对鼠白血病病毒的结构抗原的同种抗血清和异源抗血清，被测试与p53的反应性。除甲氨蝶呤抗血清外，仅体外制备的抗另一种BALB/c肉瘤CMS 4的抗血清具有抗p53活性。测试的所有转化的小鼠细胞表达p53。这些试验包括化学诱导的肉瘤、白血病、自发转化的成纤维细胞和由SV 40和鼠肉瘤病毒转化的细胞。p53在未知病毒病因的肿瘤中的存在表明由常驻细胞基因编码。这并不排除内源性病毒作为编码序列的来源或转化病毒直接编码p53的可能性。
Antisera prepared against BALB/c Meth A sarcoma in syngeneic or compatible F1 mice recognized a protein with an apparent MW of 53,000 in extracts of [35S]methionine-labeled transformed BALB/c cells. This component, designated p53, was not detected in normal adult mouse fibroblasts, lymphoid cells or hematopoietic cells or in mouse embryo cells or 3T3 [fibroblasts] cells. An extensive variety of antisera, including alloantisera and heterologous antisera directed against structural antigens of murine leukemia viruses, was tested for reactivity with p53. Other than Meth A antisera, only comparably prepared antisera against another BALB/c sarcoma, CMS4, had anti-p53 activity. All transformed mouse cells tested expressed p53. The tests included chemically induced sarcomas, leukemias, spontaneously transformed fibroblasts and cells transformed by SV40 and murine sarcoma virus. The presence of p53 in tumors of no known viral etiology indicated coding by resident cellular genes. This does not exclude endogenous viruses as the source of coding sequences or the possibility that transforming viruses code directly for p53.