Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection
Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection
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DOI：
10.1016/j.jviromet.2006.11.001
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发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Chua, K. B.
中科院分区：
文献类型：
--
作者：
Kumarasamy, V.;Wahab, A. H. Abdul;Chua, K. B.
评价了商业化的登革NSI抗原捕获ELISA，以证明其用于急性登革病毒感染的早期实验室诊断的潜在应用。213例实验室确诊的急性登革病毒感染者血清标本中199例检出登革病毒NSI抗原，而354例健康献血员血清标本均未检出。登革热NSI抗原捕获ELISA的总体灵敏度为93.4%（19/213），特异性为100%（354/354）。急性原发性登革热的敏感性（97.3%）显著高于急性继发性登革热（70.0%）。登革NS I抗原捕获ELISA法的阳性预测值为100%，阴性预测值为97.3%，病毒分离法的总阳性分离率为68.1%，其中急性原发性登革和急性继发性登革的阳性分离率分别为73.9%和31.0%。RT-PCR检测登革RNA的总阳性检出率为66.7%，对急性原发性登革和急性继发性登革的检出率分别为65.2%和75.9%，表明商业化登革NSI抗原捕获ELISA法在单份血清的实验室诊断中上级病毒分离和RT-PCR。(c)2006 Elsevier B. V.保留所有权利。
A commercial dengue NSI antigen-capture ELISA was evaluated to demonstrate its potential application for early laboratory diagnosis of acute dengue virus infection. Dengue Virus NSI antioen was detected in 199 of 213 acute serum samples from patients with laboratory confirmation of acute dengue virus infection but none of the 354 healthy blood donors' serum specimens. The dengue NSI antigen-capture ELISA gave an overall sensitivity of 93.4% (19/213) and a specificity of 100% (354/354). The sensitivity was significantly higher in acute primary dengue (97.3%) than in acute secondary dengue (70.0%). The positive predictive value of the dengue NS I antigen-capture ELISA was 100% and negative predictive value was 97.3%.Comparatively, virus isolation gave an overall positive isolation rate of 68.1% with a positive isolation rate of 73.9 and 31.0% for acute primary dengue and acute secondary dengue, respectively. Molecular detection of dengue RNA by RT-PCR gave an overall positive detection rate of 66.7% with a detection rate of 65.2 and 75.9% for acute primary dengue and acute secondary dengue, respectively.The results indicate that the commercial dengue NSI antigen-capture ELISA may be superior to virus isolation and RT-PCR for the laboratory diagnosis of acute dengue infection based on a single serum sample. (c) 2006 Elsevier B.V. All rights reserved.