KChIP1 and frequenin modify shal-evoked potassium currents in pyloric neurons in the lobster stomatogastric ganglion.
KChIP1 and frequenin modify shal-evoked potassium currents in pyloric neurons in the lobster stomatogastric ganglion.
复制标题
KChIP1 和 freequenin 改变龙虾口胃神经节幽门神经元中 shal 诱发的钾电流。
DOI：
10.1152/jn.00837.2002
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Harris-Warrick,RM
中科院分区：
文献类型：
--
作者：
Zhang,Y;MacLean,JN;An,WF;Lanning,CC;Harris-Warrick,RM
The transient potassium current (IA) plays an important role in shaping the firing properties of pyloric neurons in the stomatogastric ganglion (STG) of the spiny lobster,Panulirus interruptus. Theshalgene encodesIAin pyloric neurons. However, when we over-expressed the lobster Shal protein byshalRNA injection into the pyloric dilator (PD) neuron, the increasedIAhad somewhat different properties from the endogenousIA. The recently cloned K-channel interacting proteins (KChIPs) can modify vertebrate Kv4 channels in cloned cell lines. When we co-expressedhKChIP1with lobstershalinXenopusoocytes or lobster PD neurons, they produced A-currents resembling the endogenousIAin PD neurons; compared with currents evoked byshalalone, their voltage for half inactivation was depolarized, their kinetics of inactivation were slowed, and their recovery from inactivation was accelerated. We also co-expressedshalin PD neurons with lobsterfrequenin, which encodes a protein belonging to the same EF-hand family of Ca2+sensing proteins ashKChIP. Frequenin also restored most of properties of theshal-evoked currents to those of the endogenous A-currents, but the time course of recovery from inactivation was not corrected. These results suggest that lobster shal proteins normally interact with proteins in the KChIP/frequenin family to produce the transient potassium current in pyloric neurons.
