The generation of prostaglandins by human lung and their effects on airway function in man.
The generation of prostaglandins by human lung and their effects on airway function in man.
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人肺产生前列腺素及其对人体气道功能的影响。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
S. Holgate
中科院分区：
文献类型：
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作者：
C. Robinson;S. Holgate
含有 5-10% 肥大细胞的人分散肺细胞通过 IgE 依赖性激活或离子载体 A23187 激发，合成并释放大量前列腺素 D2 (PGD2) 和血栓素 B2 (TXB2)。这些前列腺素的产生和组胺的释放与激活刺激的强度有关。激活后，组胺和 PGD2 的释放同时进行，这些介质的释放之间的显着相关性表明它们具有共同的肥大细胞起源。使用 Percoll 密度梯度的细胞富集实验支持了这一假设。前列腺素D2和组胺的释放总是与含有肥大细胞的部分相关，而TXB2的产生主要与单核细胞-巨噬细胞系列的细胞相关。推定的 5-脂氧合酶抑制剂 6,9-deepoxy-6, 9-(苯基亚氨基)- 6,8-前列腺素 I1（U-60,257，Piriprost）对离子载体激活的人肺细胞产生免疫反应性白三烯 C4 具有复杂的抑制和增强作用。该药物还对 PGD2 释放具有令人惊讶的增强作用，同时抑制 TXB2 的生成。讨论了人肺细胞中前列腺素的释放与前列腺素在哮喘中的假定作用，特别强调 PGD2 对人体内气道功能的药理作用。
Human dispersed lung cells containing 5-10% mast cells synthesised and released large quantities of prostaglandin D2 (PGD2) and thromboxane B2 (TXB2), with either IgE-dependent activation or ionophore A23187 challenge. The generation of these prostanoids and the release of histamine was related to the strength of activation stimulus. After activation the release of histamine and PGD2 proceeded in parallel, and the significant correlation between the release of these mediators suggests a common mast cell origin. This hypothesis is supported by cell enrichment experiments using Percoll density gradients. Prostaglandin D2 and histamine release was always associated with those fractions containing mast cells, whereas the generation of TXB2 was mainly associated with cells of the monocyte-macrophage series. The putative 5-lipoxygenase inhibitor 6,9-deepoxy-6, 9-(phenylimino)- 6,8-prostaglandin I1 (U-60,257, Piriprost) had complex inhibitory and potentiating effects on immunoreactive leukotriene C4 generation from ionophore activated human lung cells. The drug also had a surprising potentiating action on PGD2 release, while simultaneously inhibiting the generation of TXB2. The release of prostanoids from human lung cells is discussed in relation to the putative role of prostaglandins in asthma, with particular emphasis on the pharmacological actions of PGD2 on human airway function in vivo.