Arsenic oxidation and bioaccumulation by the acidophilic protozoan, Euglena mutabilis, in acid mine drainage (Carnoulès, France).
Arsenic oxidation and bioaccumulation by the acidophilic protozoan, Euglena mutabilis, in acid mine drainage (Carnoulès, France).
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酸性矿山排水中嗜酸性原生动物眼虫的砷氧化和生物累积（卡努勒斯，法国）。
DOI：
10.1016/j.scitotenv.2003.08.004
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发表时间：
2004
影响因子：
9.8
通讯作者：
F. Elbaz
中科院分区：
文献类型：
--
作者：
C. Casiot;O. Bruneel;J. Personné;M. Leblanc;F. Elbaz
在卡努雷·S尾矿的酸流中(pH 2.5~4.7)，嗜酸原生动物变形真甲藻的生长具有极高的硫酸盐(1.9~4.9g/L)、铁(0.7~1.7g/L)和砷(0.08~0.26g/L)浓度。在初冬，流速变化很大，硫酸盐浓度高达4.9g/L，这可能是2001年10月至12月原生动物细胞数量减少的原因。溪流中原生动物的砷浓度和/或形态与丰度之间没有建立任何关系。亚砷酸盐是毒性最强的形式，主要存在于水中。在实验室实验中，当Reigous Creek水和合成的富As(III)培养基中存在变形杆菌时，亚砷酸盐在几天内就会氧化成砷酸盐。在培养基中未检测到甲基化化合物(MMA、DMA)。原生动物在细胞内积累As(33 6±112μg As/g干重)。无机亚砷酸盐(10 5±5 2μg As/g干重)砷盐(2 31±112μg As/g干重)。在250 mg/L As(III)合成培养基中生长的E.mutabilis对As(V)的细胞表面吸附量达到57 mg/g干重。随着培养液中As(III)浓度的增加，细胞内的积累和细胞表面的吸附都增加，但细胞内相对于可溶性As的浓缩系数降低。
In the acid stream (pH 2.5–4.7) originating from the Carnoulès mine tailings, the acidophilic protozoan Euglena mutabilis grows with extremely high sulfate (1.9–4.9 g/l), iron (0.7–1.7 g/l) and arsenic concentrations (0.08–0.26 g/l). Strong variations in flow rate and high sulfate concentrations (up to 4.9 g/l) have been registered in early winter and might be the reason for the reduction in cell number of the protozoan from October to December 2001. No relation was established between arsenic concentration and/or speciation and abundance of the protozoan in the stream. Arsenite, which is the most toxic form, predominates in water. The oxidation of arsenite to arsenate occurred within a few days in laboratory experiments when E. mutabilis was present in Reigous Creek water and synthetic As(III)-rich culture medium. Methylated compounds (MMA, DMA) were not identified in the culture media. The protozoan bioaccumulated As in the cell (336±112 μg As/g dry wt.) as inorganic arsenite (105±52 μg As/g dry wt.) and arsenate (231±112 μg As/g dry wt.). Adsorption of As at the cell surface reached 57 mg/g dry wt. in the As(V) form for E. mutabilis grown in 250 mg/l As(III) synthetic medium. Both intracellular accumulation and adsorption at the cell surface increased for increasing As(III) concentration in the medium but the concentration factor in the cell relative to soluble As decreased.