Polyphenol-containing azuki bean (Vigna angularis) extract attenuates blood pressure elevation and modulates nitric oxide synthase and caveolin-1 expressions in rats with hypertension
Polyphenol-containing azuki bean (Vigna angularis) extract attenuates blood pressure elevation and modulates nitric oxide synthase and caveolin-1 expressions in rats with hypertension
复制标题
DOI：
10.1016/j.numecd.2008.09.007
复制
发表时间：
2009-09-01
影响因子：
3.9
通讯作者：
Sato, S.
中科院分区：
文献类型：
--
作者：
Mukai, Y.;Sato, S.
背景和目的：小豆(Vigna Angularis)含有原花青素等多酚类物质，具有潜在的自由基清除活性。本实验观察了含多酚红豆提取物(ABE)对慢性高血压大鼠血压升高、一氧化氮(NO)生成及主动脉和肾脏内皮细胞一氧化氮合酶(ENOS)、诱导型一氧化氮合酶(INOS)和小窝蛋白-1蛋白表达的影响。方法与结果：自发性高血压大鼠(SHR/LZM)，血压约200 mm Hg(SBP)，随机分为0%和0.9%两组。以年龄匹配的血压正常的Wistar-京都大鼠作为对照。测定24 h尿硝酸盐/亚硝酸盐(NOx)排泄量以评估NO的生成。治疗8周后，采用免疫印迹法检测大鼠主动脉和肾脏eNOS、iNOS和小窝蛋白-1的表达。经ABE治疗的SHR的SBP明显低于未治疗的SHR。经ABE治疗的SHR 24 h尿NOx排泄量显著高于未治疗的SHR。未治疗的SHR大鼠主动脉和肾脏eNOS和iNOS的表达明显上调，而ABE治疗的SHR大鼠主动脉和肾脏eNOS和iNOS的表达受到抑制。血管和肾脏。Abe治疗后SHR小窝蛋白-1表达上调。结论：Abe可降低高血压大鼠的血压，增加NO的产生。这可能与高血压发生发展过程中主动脉和肾脏eNOS和iNOS蛋白表达的调控有关。(C)2008爱思唯尔B.V.保留所有权利。
Background and aims: Azuki beans (Vigna angularis) contain polyphenols such as proanthocyanidins that exhibit potential radical scavenging activities. We herein investigated the effects of polyphenol-containing azuki bean extract (ABE) on elevated blood pressure, nitric oxide (NO) production, and expressions of endothelial NO synthase (eNOS), inducible NOS (iNOS), and caveolin-1 proteins in the aorta and kidney of chronically hypertensive rats.Methods and results: Spontaneously hypertensive rats (SHRs/lzm) with approximately 200 mm Hg systolic blood pressure (SBP) were randomly divided into 2 groups fed either 0% or 0.9% ABE-containing diet. Age-matched normotensive Wistar-Kyoto rats were used as the control. The content of 24-h urinary nitrate/nitrite (NOx) excretion was measured to evaluate NO production. After 8 weeks of treatment, the eNOS, iNOS, and caveolin-1 protein expressions in the aorta and kidney were analyzed by western blotting. The SBP of the ABE-treated SHR was significantly lower than that of the untreated SHR. The level of 24-h urinary NOx excretion was significantly higher in the ABE-treated SHR than in the untreated SHR. The eNOS and iNOS expressions in the aorta and kidney were remarkably upregulated in the untreated SHR but suppressed in the ABE-treated SHR. The vascular and renal. caveolin-1 expressions were upregulated in the ABE-treated SHR.Conclusions: ABE reduced the elevated blood pressure and increased NO production in long-term treatment. It may be associated with the modulation of eNOS and iNOS protein expressions in the aorta and kidney during the development of hypertension. (C) 2008 Elsevier B.V. All rights reserved.