Technologies for Single-Cell Isolation.
Technologies for Single-Cell Isolation.
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DOI：
10.3390/ijms160816897
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发表时间：
2015-07-24
影响因子：
5.6
通讯作者：
Koltay P
中科院分区：
文献类型：
--
作者：
Gross A;Schoendube J;Zimmermann S;Steeb M;Zengerle R;Koltay P
单细胞的处理在诸如细胞系开发或单细胞分析的应用中是非常重要的，例如，用于癌症研究或新兴的诊断方法。本文综述了目前使用或正在开发的分离单细胞用于后续单细胞分析的技术。为确定最相关的技术而进行的专门在线市场调查的数据首次显示，（荧光激活细胞分选）分别流式细胞术（33%的使用率），激光显微切割（17%），手动细胞挑选（17%），随机接种/稀释（15%），和微流体/芯片实验室设备（12%）是目前最常用的技术。这些最突出的技术进行了详细描述，并讨论了关键的性能因素。调查数据表明，未来几年对单细胞分离工具的兴趣进一步增加。此外，还进行了全球专利检索，以筛选未来可能相关的新兴技术。共发现179项专利，其中25项通过筛选与该领域相关的标题和摘要进行了评估。
The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to identify the most relevant technologies, presented here for the first time, shows that FACS (fluorescence activated cell sorting) respectively Flow cytometry (33% usage), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip devices (12%) are currently the most frequently used technologies. These most prominent technologies are described in detail and key performance factors are discussed. The survey data indicates a further increasing interest in single-cell isolation tools for the coming years. Additionally, a worldwide patent search was performed to screen for emerging technologies that might become relevant in the future. In total 179 patents were found, out of which 25 were evaluated by screening the title and abstract to be relevant to the field.