Human nasal epithelial cell culture system: evaluation of response to human interferons
Human nasal epithelial cell culture system: evaluation of response to human interferons
复制标题
人鼻上皮细胞培养系统：评估对人干扰素的反应
作者：
M. Harmon;S. Greenberg;P. E. Johnson;R. Couch
本报告描述了人鼻上皮细胞对外源性人干扰素（IF）的反应。为了测量反应，使用了一种体外研究人鼻上皮细胞的系统。鼻上皮细胞对白细胞和成纤维细胞IF的反应相同，对病毒复制的抗性发展是时间和剂量依赖的。细胞需要与1,000 U IF（400 69/19参考单位）接触4小时，才能持续显著降低病毒产量。该体外细胞培养系统可用于预测人IF对呼吸道病毒感染的体内反应。
This report describes the response of human nasal epithelial cells to exogenously applied human interferon (IF). To measure the response, a system developed to study human nasal epithelial cells in vitro was used. Nasal epithelial cells responded equally to both leukocyte and fibroblast IF, with development of resistance to virus replication that was time and dose dependent. Cells required contact with 1,000 U of IF (400 69/19 reference units) for 4 h to produce consistently a significant reduction in virus yield. This in vitro cell cultured system may be useful in predicting the in vivo response of human IF in respiratory viral infections.