NCAM-dependent neurite outgrowth is inhibited in neurons from Fyn-minus mice.
NCAM-dependent neurite outgrowth is inhibited in neurons from Fyn-minus mice.
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DOI：
10.1083/jcb.127.3.825
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发表时间：
1994-11
影响因子：
7.8
通讯作者：
Maness, P F
中科院分区：
文献类型：
--
作者：
Beggs, H E;Soriano, P;Maness, P F
神经生长锥（p59fyn， pp60c-src和pp62c-yes）中src相关的非受体蛋白酪氨酸激酶是细胞粘附分子导向轴突生长的潜在细胞内信号分子。为了确定src相关酪氨酸激酶是否介导NCAM依赖性的神经突生长，我们分析了fyn-、src-和yes-阴性小鼠的小脑和感觉神经元在转染ncam140的L成纤维细胞单层上的神经突生长情况。NCAM依赖性的神经突生长在fyn-小鼠的小脑和背根神经节神经元中被选择性地抑制，而src-或yes-小鼠则没有。fyn-、src-或yes-神经元在未转染的成纤维细胞单层上的神经突生长不受影响，这表明这些激酶至少在成纤维细胞上的一些整合素或其他粘附底物上对轴突生长没有显著贡献。本研究表明p59fyn是NCAM信号通路中导致轴突生长的重要组成部分。
Src-related nonreceptor protein tyrosine kinases in nerve growth cones (p59fyn, pp60c-src, and pp62c-yes) are potential intracellular signaling molecules for cell adhesion molecule-directed axonal growth. To determine whether src-related tyrosine kinases mediate NCAM- dependent neurite outgrowth, cultures of cerebellar and sensory neurons from fyn-, src-, and yes- minus mice were analyzed for neurite outgrowth on monolayers of NCAM140-transfected L fibroblasts. NCAM- dependent neurite outgrowth was selectively inhibited in cultures of cerebellar and dorsal root ganglion neurons from fyn-, but not src- or yes- mice. Neurite outgrowth by fyn-, src-, or yes- neurons on untransfected fibroblast monolayers was unaffected, indicating that these kinases do not contribute significantly to axon growth on at least some integrins or other adhesive substrates present on fibroblasts. This study demonstrates that p59fyn is an essential component of the NCAM signaling pathway leading to axonal growth.