Complete amino acid sequence of bovine colostrum low‐M r cysteine proteinase inhibitor
Complete amino acid sequence of bovine colostrum low‐M
r cysteine proteinase inhibitor
复制标题
牛初乳low-M的完整氨基酸序列
DOI：
10.1016/0014-5793(85)81335-1
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发表时间：
1985
期刊：
影响因子：
3.5
通讯作者：
S. Fujii
中科院分区：
文献类型：
--
作者：
Masayuki Hirado;S. Tsunasawa;F. Sakiyama;M. Niinobe;S. Fujii
通过对牛初乳半胱氨酸蛋白酶抑制剂天然肽段和经溴化氰降解、无色杆菌内肽酶酶解、还原和S-羧甲基化蛋白部分酸水解得到的肽段进行序列测定，确定了牛初乳半胱氨酸蛋白酶抑制剂的氨基酸序列，并成功地用无色杆菌内肽酶酶解得到了两个含二硫键的肽段。该抑制剂由112个氨基酸组成，Mr为12787。在Cys 66和Cys 77之间以及Cys 90和Cys 110之间建立了两个二硫键。与人γ-trace、人唾液酸蛋白和鸡卵清cystatin的同源性较高。
The complete amino acid sequence of bovine colostrum cysteine proteinase inhibitor was determined by sequencing native inhibitor and peptides obtained by cyanogen bromide degradation,Achromobacterlysylendopeptidase digestion and partial acid hydrolysis of reduced andS-carboxymethylated protein.Achromobacterpeptidase digestion was successfully used to isolate two disulfide-containing peptides. The inhibitor consists of 112 amino acids with anMrof 12787. Two disulfide bonds were established between Cys 66 and Cys 77 and between Cys 90 and Cys 110. A high degree of homology in the sequence was found between the colostrum inhibitor and human γ-trace, human salivary acidic protein and chicken egg-white cystatin.
α2-硫醇蛋白酶抑制剂的人 cDNA 的分离及其与低分子量激肽原的同一性。
影响因子：
2.9
作者：
Ohkubo,I;Kurachi,K;Takasawa,T;Shiokawa,H;Sasaki,M
通讯作者：
Sasaki,M