FLUORESCENT-ANTIBODY STAINING METHOD FOR ENUMERATION OF VIABLE ENVIRONMENTAL VIBRIO-CHOLERAE 01
FLUORESCENT-ANTIBODY STAINING METHOD FOR ENUMERATION OF VIABLE ENVIRONMENTAL VIBRIO-CHOLERAE 01
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DOI：
10.1016/0167-7012(87)90015-7
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发表时间：
1987-09-01
影响因子：
2.2
通讯作者：
COLWELL, RR
中科院分区：
文献类型：
--
作者：
BRAYTON, PR;COLWELL, RR
本文介绍了一种用免疫荧光染色法测定环境水样中霍乱弧菌01活菌数的薄膜过滤法。将样品与酵母提取物和萘啶酸一起孵育。底物反应性细胞，即活细胞，伸长，并且在用特异性抗血清和荧光素缀合物染色后，当在显微镜下观察时，活的霍乱弧菌细胞表现为长的外周荧光绿色带杆菌。使用目镜分划板，可以计算每ml活细胞的数量。如果使用特异性抗血清，则该程序已适用于外部细菌菌种。
A membrane filtration method has been developed which is useful for enumeration of viable Vibria cholerae 01 in environmental water samples by immunofluorescent staining. The samples were incubated with yeast extract and nalidixic acid. Substrate responsive cells, i.e. viable cells, elongate and after staining with specific antiserum and fluorescein conjugate, viable V. cholerae cells appear as long, peripheral fluorescent green banded bacilli when viewed under the microscope. Using an ocular reticule, the number of viable cells per ml can be calculated. The procedure has been adapted for use with outer bacterial species if specific antisera are employed.