Characteristics of core promoter and precore stop codon mutants of hepatitis B virus in Vietnam
Characteristics of core promoter and precore stop codon mutants of hepatitis B virus in Vietnam
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DOI：
10.1002/jmv.20175
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发表时间：
2004-10-01
影响因子：
12.7
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Huy, TTT;Ushijima, H;Abe, K
在亚洲，B型和C型是最常见的乙肝病毒基因型别，而C型型别会导致更严重的肝病。核心启动子/前核心(CP/PC)突变，已知与这些基因相关，可能会对肝病的进展和严重程度产生影响。对115例越南患者，其中急性感染者39例，慢性感染者76例进行了肝功能、HBeAg、基因分型和HBVDNA水平的检测。对CP/PC的282个核苷酸片段进行了扩增、测序和分析。急性期组CP/PC突变分别占38.4%和25.6%。B型占主导地位(74.3%，P<；0.05)，并与PC突变(A1896)连锁(P<；0.05)。慢性组中CP/PC突变分别占61.7%和32.8%。CP突变，尤其是T1762/A1764双突变，被发现与C型(81%，P<0.001)、肝硬变和肝细胞癌(P<0.05)相关。因此，越南人群中C-1858基因携带率较高(70%)的C基因可能在严重慢性肝病的发病中起重要作用。
In Asia, genotypes B and C are the most common genotypes of hepatitis B virus (HBV); and genotype C causes more severe liver disease. Core promoter/precore (CP/PC) mutants, known to be linked to these genotypes, could have an impact on the progression and severity of liver disease. Sera of 115 patients, including 39 acute and 76 chronic Vietnamese HBV infected patients, were tested for their liver profile, HBeAg, HBV genotypes, and HBV DNA level. Fragments of 282 nucleotides covering CP/PC were amplified, sequenced, and analysed. In the acute group, CP/PC mutants accounted for 38.4 and 25.6%, respectively. Genotype B was found to be predominant (74.3%, P < 0.05) and linked to the PC mutant (A1896) (P < 0.05). In the chronic group, CP/PC mutants accounted for 61.7 and 32.8%. CP mutants, especially the T1762/A1764 double mutant, were found to correlate with genotype C (81%, P < 0.001), liver cirrhosis, and hepatocellular carcinoma (P < 0.05). Therefore, genotype C in Vietnam, which carried high rate of C-1858 (70%), could play an important role in causing severe chronic liver disease.