Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor
Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor
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DOI：
10.2141/jpsa.010102
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发表时间：
2011-01-01
影响因子：
1.5
通讯作者：
Horiuchi, Hiroyuki
中科院分区：
文献类型：
--
作者：
Nakano, Mikiharu;Arisawa, Kenjiro;Horiuchi, Hiroyuki
鸡是发育生物学理想的动物模型研究工具，是一种具有优良生产力的农场动物。因此，研究人员长期以来一直在寻求建立鸡胚胎干细胞（cESCs），以培育转基因鸡。在这里，我们从鸡白血病抑制因子（chLIF）培养的鸡胚皮细胞（CBCs）中获得了新的cESCs。这些cESCs具有长期（100天或更长时间）连续传代的能力，能够表达鸡Nanog （chNanog）和鸡vasa同源（Cvh） mrna和蛋白。在使用受精卵的移植实验中，cESCs显示出嵌合形成的能力。将增强的绿色荧光蛋白（EGFP）基因转移到cESCs中，可以在原始生殖细胞（PGCs）中观察到绿色荧光。这些结果表明，新型cESCs应该具有向生殖细胞分化的能力。
Chickens are an ideal animal model study tool for developmental biology, and a farm animal with excellent productivity. Researchers have therefore long sought to establish chicken embryonic stem cells (cESCs) to enable the creation of genetically modified chickens. Here, we derived novel cESCs from chicken blastodermal cells (CBCs) cultured with chicken leukemia inhibitory factor (chLIF). These cESCs have the capacity for long-term (100 days or more) successive subculture and express both chicken Nanog (chNanog) and chicken vasa homolog (Cvh) mRNAs and proteins. The cESCs showed a capacity for chimeric formation during a transplant experiment that used a fertilized egg. Transfer of the enhanced green fluorescent protein (EGFP) gene to cESCs enabled green fluorescence to be observed among primordial germ cells (PGCs). These results indicate that novel cESCs should have the capacity to differentiate into germ cells.