Studying Membrane Topology of the Neuromuscular Junction by Electron Tomography of Thick Resin Slices in a Cs/Cc-corrected TEM
Studying Membrane Topology of the Neuromuscular Junction by Electron Tomography of Thick Resin Slices in a Cs/Cc-corrected TEM
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通过 Cs/Cc 校正 TEM 中厚树脂切片的电子断层扫描研究神经肌肉接头的膜拓扑
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发表时间：
2010
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通讯作者：
R. Schröder
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作者：
I. Wacker;Iv Roeder;R. Rudolf;S. Hillmer;B. Kabius;G. Hofhaus;R. Schröder
整个细胞或大体积组织样品的电子断层扫描对于在其自然细胞环境中的大分子复合体的成像具有重要意义。为了克服繁琐和技术困难的连续切片，我们想要成像相当厚的切片。到目前为止，由于电子显微镜的大色差(CC)效应，厚样品的成像受到了阻碍。主要的多重非弹性散射会导致图像模糊，因为不同能量(“颜色”)的电子会在不同的像平面上成像。随着最近新的、像差校正的光学显微镜的问世(在团队项目[1]的框架内)，我们能够记录来自异常厚的肌肉组织切片的高分辨率断层图像，这是这种新型显微镜的第一个生物学应用。
Electron Tomography of whole cells or large volume tissue samples is of central importance for the imaging of macromolecular complexes in their native cellular environment. To overcome the tedious and technically difficult serial sectioning we wanted to image rather thick sections. So far imaging of thick samples was hampered by the effects of the large chromatic aberration (Cc) of TEMs. The dominant multiple inelastic scattering leads to blurred images, since electrons at different energies (“colours”) will be imaged in different image planes. With the recent availability of novel, aberration-corrected TEMs (within the framework of the TEAM project [1]) we were able to record high-resolution tomograms from unusually thick sections of muscle tissue as a first biological application of this novel microscope.