Medium transitory oxygen-glucose deprivation induced both apoptosis and necrosis in cerebellar granule cells
Medium transitory oxygen-glucose deprivation induced both apoptosis and necrosis in cerebellar granule cells
复制标题
DOI：
10.1016/s0304-3940(97)00914-2
复制
发表时间：
1998-01-02
影响因子：
2.5
通讯作者：
Zharkovsky, A
中科院分区：
文献类型：
--
作者：
Kalda, A;Eriste, E;Zharkovsky, A
最近的实验表明，缺血性损伤可以诱导坏死和凋亡。本研究旨在探讨缺氧缺糖（OGD）对小脑颗粒细胞凋亡的诱导作用。培养基OGD（90分钟）诱导细胞培养物中的细胞凋亡，暴露后12小时效果最大，如以下形态学（TdT介导的dUTP-生物素缺口末端标记）和生化标记（DNA寡核小体片段化）所示。线粒体损伤（MTT法）是我们在OGD期间和之后检测到的早期效应之一，并且与TUNEL阳性细胞的动力学相关。LDH释放量从受损细胞，与坏死后12小时显着增加。这些结果表明，培养基OGD诱导快速（
Recent experiments have shown that an ischemic insult can induce both necrosis and apoptosis. A series of experiments were designed to examine the potential induction of apoptosis by oxygen-glucose deprivation (OGD) in cerebellar granule cell culture. A medium OGD (90 min) induced apoptosis in cell culture, with maximal effect 12 h after exposure, as indicated by following morphological (TdT-mediated dUTP-biotin nick end-labeling) and biochemical markers (DNA oligonucleosomal fragmentation). Mitochondrial injury (MTT assay) was among the early effects we detected during and after OGD and it was correlated with the dynamics of TUNEL positive cells. The amount of LDH release from damaged cells, associated with necrosis was increased significantly 12 h after exposure. These results indicate that medium OGD induced a rapid (