Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration
Enhanced angiogenesis through controlled release of basic fibroblast growth factor from peptide amphiphile for tissue regeneration
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DOI：
10.1016/j.biomaterials.2006.08.003
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发表时间：
2006-12-01
期刊：
影响因子：
14
通讯作者：
Tabata, Yasuhiko
中科院分区：
文献类型：
--
作者：
Hosseinkhani, Hossein;Hosseinkhani, Mohsen;Tabata, Yasuhiko
在本研究中，我们假设一种促进血管生成的新方法将是创建可注射的三维(3D)支架，该支架包裹生长因子，促进生长因子的持续释放并诱导血管生成。我们证明，多肽-两亲性(PA)水溶液与碱性成纤维细胞生长因子(BFGF)悬浮液混合可以形成三维支架。PA是通过标准固相化学合成的，以NH2末端的烷基化为终点。将碱性成纤维细胞生长因子悬浮液与PA的稀水溶液混合，形成三维纳米纤维网络。扫描电子显微镜(SEM)观察发现，纤维集合体的形成具有极高的长径比和高比表面积。在体外和体内研究了碱性成纤维细胞生长因子从三维纳米纤维网络中的释放情况，并对释放的碱性成纤维细胞生长因子诱导的血管生成进行了评估。在小鼠背部皮下注射PA水溶液和bFGF混悬液时，注射部位形成透明的三维水凝胶，并在注射部位周围诱导明显的血管生成，与单独注射bFGF或PA形成明显的对比。联合应用碱性成纤维细胞生长因子诱导的血管生成是一种很有前途的促进组织再生的方法。(C)2006爱思唯尔有限公司。保留所有权利。
In the present study, we hypothesized that a novel approach to promote vascularization would be to create injectable three-dimensional (3-D) scaffolds with encapsulated growth factor that enhance the sustained release of growth factor and induce the angiogenesis. We demonstrate that a 3-D scaffold can be formed by mixing of peptide-amphiphile (PA) aqueous solution with basic fibroblast growth factor (bFGF) suspension. PA was synthesized by standard solid phase chemistry that ends with the alkylation of the NH2 terminus of the peptide. A 3-D network of nanofibers was formed by mixing bFGF suspensions with dilute aqueous solutions of PA. Scanning electron microscopy (SEM) observation revealed the formation of fibrous assemblies with an extremely high aspect ratio and high surface areas. In vitro and in vivo release profile of bFGF from 3-D network of nanofibers was investigated while angiogenesis induced by the released bFGF was assessed. When aqueous solution of PA was subcutaneously injected together with bFGF suspension into the back of mice, a transparent 3-D hydrogel was formed at the injected site and induced significant angiogenesis around the injected site, in marked contrast to bFGF injection alone or PA injection alone. The combination of bFGF-induced angiogenesis is a promising procedure to improve tissue regeneration. (c) 2006 Elsevier Ltd. All rights reserved.