Chemical and optical control of CRISPR-associated nucleases.
Chemical and optical control of CRISPR-associated nucleases.
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DOI：
10.1016/j.cbpa.2020.10.003
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发表时间：
2021-02-01
影响因子：
7.8
通讯作者：
Choudhary, Amit
中科院分区：
文献类型：
--
作者：
Modell, Ashley E;Siriwardena, Sachini U;Choudhary, Amit
细菌的聚集规律间隔短回文重复序列(CRISPR)-Cas系统提供了可编程核酸酶（例如Cas9），这些酶正在改变基因组编辑领域的应用，在基础和生物医学研究、生物技术和农业领域。然而，Cas9在现实世界的广泛应用需要精确控制其剂量、时间和空间上的活性，因为脱靶效应、胚胎嵌合体、染色体易位和遗传毒性已被观察到与核酸酶活性升高和/或延长有关。在这里，我们回顾了精确控制Cas9活性的化学和光学方法。
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system of bacteria has furnished programmable nucleases (e.g., Cas9) that are transforming the field of genome editing with applications in basic and biomedical research, biotechnology, and agriculture. However, broader real-world applications of Cas9 require precision control of its activity over dose, time, and space as off-target effects, embryonic mosaicism, chromosomal translocations, and genotoxicity have been observed with elevated and/or prolonged nuclease activity. Here, we review chemical and optical methods for precision control of Cas9's activity.