Feedback regulation is central to Delta-Notch signalling required for Drosophila wing vein morphogenesis.
Feedback regulation is central to Delta-Notch signalling required for Drosophila wing vein morphogenesis.
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发表时间：
1997-09
期刊：
影响因子：
4.6
通讯作者：
S. Huppert;T. Jacobsen;M. Muskavitch
中科院分区：
文献类型：
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作者：
S. Huppert;T. Jacobsen;M. Muskavitch
在果蝇的变态过程中，Delta和Notch是静脉和静脉间细胞命运分配所必需的。我们发现，在25℃下蛹期形成22至30小时后，这些细胞的分配依赖于Delta介导的信号传导。在provein中，Delta在中央provein细胞（假定的静脉细胞）中表达得更高，而Notch在外侧provein细胞（假定的静脉间细胞）中表达得更高。Notch在假定的静脉间细胞中的积累依赖于假定的静脉细胞中的Delta信号活性，而构成型Notch受体活性抑制假定静脉细胞中的Delta积累。当Delta蛋白在假定的脉间细胞中表达异常升高时，原静脉细胞中互补的Delta和Notch表达模式被逆转，并且由于中央原静脉细胞无法稳定地接受静脉细胞的命运而发生静脉丢失。我们的研究结果表明，在变质翼静脉发育过程中，Delta-Notch信号对Delta和Notch的表达进行了反馈调节，由此产生的Delta和Notch表达的不对称性奠定了provein中静脉和脉间细胞命运的适当规范。
Delta and Notch are required for partitioning of vein and intervein cell fates within the provein during Drosophila metamorphosis. We find that partitioning of these fates is dependent on Delta-mediated signalling from 22 to 30 hours after puparium formation at 25 degrees C. Within the provein, Delta is expressed more highly in central provein cells (presumptive vein cells) and Notch is expressed more highly in lateral provein cells (presumptive intervein cells). Accumulation of Notch in presumptive intervein cells is dependent on Delta signalling activity in presumptive vein cells and constitutive Notch receptor activity represses Delta accumulation in presumptive vein cells. When Delta protein expression is elevated ectopically in presumptive intervein cells, complementary Delta and Notch expression patterns in provein cells are reversed, and vein loss occurs because central provein cells are unable to stably adopt the vein cell fate. Our findings imply that Delta-Notch signalling exerts feedback regulation on Delta and Notch expression during metamorphic wing vein development, and that the resultant asymmetries in Delta and Notch expression underlie the proper specification of vein and intervein cell fates within the provein.