Label-free ultrarapid spheroid formation in microfluidic chip using magneto-Archimedes effect
Label-free ultrarapid spheroid formation in microfluidic chip using magneto-Archimedes effect
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DOI：
10.1109/memsys.2012.6170107
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
Y. Akiyama;K. Morishima
中科院分区：
文献类型：
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作者：
Y. Akiyama;K. Morishima
本文演示了在微流控芯片中既没有细胞标记也没有特定微通道图案的球体阵列的形成。无标记的超快速磁性细胞操作是通过向培养基中添加顺磁性盐来增强细胞的抗磁性而实现的。通过施加磁场，顺磁性介质中的细胞在微通道内的阵列上超快速聚集，并且细胞聚集体在培养一天后变成球状体。结果表明，这种细胞操作方法是一种有前途的方法来构建微型三维组织在一个封闭的小空间。
This paper demonstrates formation of a spheroid array in a microfluidic chip with neither cell labeling nor a specific microchannel pattern. Label-free ultrarapid magnetic cell manipulation is made possible by adding a paramagnetic salt to the culture medium to enhance the diamagnetic property of the cells. The cells in the paramagnetic medium aggregate ultrarapidly on an array within a microchannel by applying a magnetic field and the cell aggregates become spheroids after one day of culture. The results suggest that this cell manipulation method is a promising approach to construct micro 3D tissues in a closed small space.