Within-host variation of avian influenza viruses
Within-host variation of avian influenza viruses
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DOI：
10.1098/rstb.2009.0088
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发表时间：
2009-09-27
影响因子：
6.3
通讯作者：
McCauley, John W.
中科院分区：
文献类型：
--
作者：
Iqbal, Munir;Xiao, Hiaxia;McCauley, John W.
H5N1禽流感病毒的出现和从亚洲到欧洲和非洲的传播造成了严重的动物疾病问题，并引起了人们对该病毒可能对人类构成大流行威胁的担忧。影响该病毒广泛分布的动物流行因素很复杂，目前流行的病毒种类反映了这些因素。病毒基因的序列分析揭示了H5N1病毒在出现和传播过程中的演变，但只有少数研究描述了单个受感染鸟类水平上的病毒变异程度。在这里，我们描述了一项研究的一些结果，其中火鸡，鸭和鸡感染两种H5N1病毒之一或三种H7N1病毒之一，并在单个感染的禽类宿主内的序列变异程度进行了检查。我们为这项工作开发了“深度扩增子”序列分析，方法和结果为该领域疾病暴发的应用提供了背景框架。
The emergence and spread of H5N1 avian influenza viruses from Asia through to Europe and Africa pose a significant animal disease problem and have raised concerns that the virus may pose a pandemic threat to humans. The epizootological factors that have influenced the wide distribution of the virus are complex, and the variety of viruses currently circulating reflects these factors. Sequence analysis of the virus genes sheds light on the H5N1 virus evolution during its emergence and spread, but the degree of virus variation at the level of an individual infected bird has been described in only a few studies. Here, we describe some results of a study in which turkeys, ducks and chickens were infected with either one of two H5N1 or one of three H7N1 viruses, and the degree of sequence variation within an individual infected avian host was examined. We developed 'deep amplicon' sequence analysis for this work, and the methods and results provide a background framework for application to disease outbreaks in the field.