Metabolomics analysis for skin ulceration syndrome of Apostichopus ja ponicus based on UPLC/Q-TOF MS
Metabolomics analysis for skin ulceration syndrome of Apostichopus ja ponicus based on UPLC/Q-TOF MS
复制标题
基于UPLC/Q-TOF MS的仿刺参皮肤溃疡综合征代谢组学分析
DOI：
10.1007/s00343-020-0205-4
复制
发表时间：
2021
影响因子：
1.6
通讯作者：
Jun Ding
中科院分区：
文献类型：
--
作者：
Yang Zhang;Youquan Wang;Xiaoyu Liu;Beichen Ding;Yi Sun;Yaqing Chang;Jun Ding
Skin ulceration syndrome (SUS) is the main diseases affected the development of sea cucumber (Apostichopus japonicus) culture industries.To better observe the changes in the sea cucumber A.japonicus with SUS and understand the pathogenesis of the disease,activities of superoxide dismutase (SOD),catalase (CAT),and level of malondialdehyde (MDA) in coelomic fluid were detected using the Assay Kit and metabolites in the body wall were assessed using ultra-performance liquid chromatography and quadrupoletime of flight mass spectrometry.The results indicated that level of MDA was increased during SUS compared with healthy individuals (P 1 and P <0.1).Pathway enrichment analysis indicated most enrichment of KEGG pathways were mainly related to energy metabolism,immunity,and osmoregulation such as ABC transporters,glycine,serine and threonine metabolism,tryptophan metabolism and neuroactive ligand-receptor interaction.Our study reflected the difference in enzyme activity and metabolites between A.japonicus with SUS and those without,which will provide reference data for investigating SUS.
