NATRIURETIC AND SODIUM-TRANSPORT INHIBITORY ACTIVITY IN PLASMA OF VOLUME-EXPANDED DOGS
NATRIURETIC AND SODIUM-TRANSPORT INHIBITORY ACTIVITY IN PLASMA OF VOLUME-EXPANDED DOGS
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DOI：
10.1038/ki.1974.2
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发表时间：
1974-01-01
影响因子：
19.6
通讯作者：
NELSON, DB
中科院分区：
文献类型：
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作者：
BUCKALEW, VM;NELSON, DB
方法雌性杂种犬，体重15 ~ 25 kg，自由进食和饮水，实验前限水12 ~ 18 h。从三组犬中采集颈静脉血浆用于生物测定：第1组。如下在16只狗中产生急性ECF扩张。戊巴比妥钠（30 mg/kg）麻醉，膀胱插管，每20 min取尿标本。当尿流达到稳定状态时（麻醉诱导后60 - 120分钟），开始以25 ml/min的速度静脉输注0.9%盐水20分钟，然后在实验的剩余时间内以10 ml/min的速度恒定输注。开始输注生理盐水后90 - 120分钟抽取75 - 100 ml血液。2组如下所述，在13只未麻醉的狗中产生急性ECF体积收缩。对犬进行称重，经口给予50 mg呋塞米，并置于代谢笼中。当观察到自发排尿时，通常在呋塞米给药后3 - 4小时，重新称重狗，记录尿量，并获得75 - 100 ml颈静脉血。第三组.在8只麻醉犬中，按如下方法产生急性血容量减少和扩张。用戊巴比妥钠（30 mg/kg）诱导麻醉，静脉注射50 mg呋塞米。通过留置的Foley导管每30 mm收集一次尿液。给予呋塞米后4小时，采集颈静脉血样并测量红细胞压积。然后加入适当体积的生理盐水，将血液稀释20 - 25%，第1组犬输注生理盐水期间的平均稀释度（见结果），并重新测量红细胞压积。然后以与第1组犬相同的方式在4只犬中产生急性容量扩张，并获得第二份血样用于红细胞压积和生物测定。在另外四只狗中，除了两只狗仅在给予呋塞米后和两只狗仅在急性容量扩张后采集血样外，遵循相同的方案，将血样抽入肝素化注射器中，立即在4 ℃分离血浆，并在-4 ℃冷冻备用。用来自Amicon Corp.的Diaflo® PM-b膜（截留摩尔重量，10，000）和型号12超滤池（10 ml体积）制备血浆超滤，马萨诸塞州的列克星敦，根据先前描述的方法[4]。简言之，在5 ℃下，使用50磅/平方英寸（psi）的氮气，以1130至1170 rpm的搅拌速度进行超滤。因为运输-
Methods Female mongrel dogs weighing 15 to 25 kg, allowed food and water ad libitum, were made hydropenic by restriction of water for 12 to 18 hours prior to an experiment. Jugular vein plasma was obtained for bioassay from three groups of dogs: Group 1. Acute ECF expansion was produced in 16 dogs as follows. Anesthesia was induced with pentobarbital sodium (30 mg/kg), the bladder catheterized and urine speci-mens collected every 20 minutes. When a steady state of urine flow had been obtained (60 to 120 mm after the induction of anesthesia), an intravenous infusion of 0.9% saline was begun at 25 ml/min for 20 minutes, followed by constant infusion of 10/mi/mm for the remainder of the experiment. Seventy-five to 100 ml of blood was drawn 90 to 120 minutes after saline infusion was begun. Group 2. Acute ECF volume contraction was produced in 13 unanesthetized dogs as follows. The dogs were weighed, given 50 mg of furosemide orally and placed in a metabolism cage. When spontaneous voiding was observed, usually three to four hours after furosemide administration, the dogs were reweighed, the volume of urine recorded and 75 to 100 ml of jugular venous blood was ob-tained. Group 3. Acute volume depletion and expan-sion was produced in eight anesthetized dogs as follows. Anesthesia was induced with pentobarbital sodium (30 mg/kg), and 50 mg of furosemide was administered intravenously. Urine was collected every 30 mm through an indwelling Foley catheter. Four hours after furosemide was administered, a jugular venous blood sample was obtained and the hematocrit was measured. An appropriate volume of saline was then added to dilute the blood 20 to 25%, the mean degree of dilution occuring during saline infusion in group 1 dogs (see Results), and the hemato-crit remeasured. In four dogs acute volume expansion was then produced in the same manner as in group 1 dogs and a second blood sample obtained for hemato-crit and bioassay. In four additional dogs, the same protocol was followed except that in two dogs blood samples were obtained only after furosemide admini-stration and in two dogs only after acute volume expansion.Blood samples were drawn into heparinized syringes and the plasma immediately separated at 4 C and frozen at—4 C until used. Ultrafiltrates of plasma were prepared with Diaflo® PM-b membranes (mol wt cutoff, 10,000) and Model 12 ultrafiltration cells (10 ml volume) from Amicon Corp., Lexington, Mass., according to methods previously described [4]. Briefly, ultrafiltration was performed at 5 C using 50 pounds per square inch (psi) of nitrogen at a stirring speed of 1130 to 1170 rpm. Because the transport-