Altered expression of TaRSL4 gene by genome interplay shapes root hair length in allopolyploid wheat
Altered expression of TaRSL4 gene by genome interplay shapes root hair length in allopolyploid wheat
复制标题
基因组相互作用改变 TaRSL4 基因的表达，影响异源多倍体小麦的根毛长度
DOI：
10.1111/nph.13615
复制
发表时间：
2016-01-01
期刊：
影响因子：
9.4
通讯作者：
Sun, Qixin
中科院分区：
文献类型：
--
作者：
Han, Yao;Xin, Mingming;Sun, Qixin
多倍体是植物进化和物种形成的主要驱动力。多倍体植物的形成、自然选择和驯化过程中经常发生表型变化。本文报道了人工合成和天然异源多倍体小麦的根毛长度显著长于其二倍体祖先的根毛长度，而异源六倍体和异源四倍体小麦的根毛长度无显著差异。在二倍体和异源四倍体小麦中，根毛长度与根长呈显著正相关。此外，在天然异源多倍体小麦中，来自A基因组的TaRSL 4同源物（TaRSL 4-A）的转录本丰度远高于其他基因组。值得注意的是，通过在小麦中过表达TaRSL 4-A增加根毛长度导致在营养贫乏的条件下增加的地上部鲜生物量。我们的观察表明，异源六倍体小麦中根毛长度的增加起源于异源四倍体祖先，并且通过基因组相互作用改变TaRSL 4基因的表达来塑造异源多倍体小麦中根毛长度。
Polyploidy is a major driving force in plant evolution and speciation. Phenotypic changes often arise with the formation, natural selection and domestication of polyploid plants. However, little is known about the consequence of hybridization and polyploidization on root hair development.Here, we report that root hair length of synthetic and natural allopolyploid wheats is significantly longer than those of their diploid progenitors, whereas no difference is observed between allohexaploid and allotetraploid wheats.The expression of wheat gene TaRSL4, an orthologue of AtRSL4 controlling the root hair development in Arabidopsis, was positively correlated with the root hair length in diploid and allotetraploid wheats. Moreover, transcript abundance of TaRSL4 homoeologue from A genome (TaRSL4-A) was much higher than those of other genomes in natural allopolyploid wheat. Notably, increased root hair length by overexpression of the TaRSL4-A in wheat led to enhanced shoot fresh biomass under nutrient-poor conditions.Our observations indicate that increased root hair length in allohexaploid wheat originated in the allotetraploid progenitors and altered expression of TaRSL4 gene by genome interplay shapes root hair length in allopolyploid wheat.