Implications of CEA and p53 overexpression in the poor prognosis of colorectal cancer
Implications of CEA and p53 overexpression in the poor prognosis of colorectal cancer
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DOI：
10.1385/mo:23:2:237
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发表时间：
2006-01-01
期刊：
影响因子：
3.4
通讯作者：
El-Hak, NG
中科院分区：
文献类型：
--
作者：
Nasif, WA;Lotfy, M;El-Hak, NG
结直肠癌（CRC）是最常见和最具侵袭性的癌症之一。一些临床病理特征已经被研究，以确定预后因素，这些因素可以提供有关结直肠癌预后好坏的信息。本研究旨在探讨血清CEA、p53表达及DNA指数与结直肠癌患者不同临床病理特征的关系。本研究纳入了50例结直肠癌患者。特异性单克隆抗体免疫组织化学检测P53蛋白。采用流式细胞术检测样品的DNA指数。采用ELISA法测定血清CEA。结果显示，27/50（54%）的患者p53阳性。在CEA反应性方面，35/50（70%）对CEA有反应性。这些结果表明CEA对结直肠癌的检测比p53更敏感。复发组与非复发组在CRC的Duke分期、生存时间、血清CEA方面差异有统计学意义（p分别= 0.001、0.016、< 0.001）。Kaplan-Meier法和log-rank检验显示，p53和CEA同时阳性的患者的平均生存时间与CEA单独阳性、p53单独阳性、p53和CEA同时阴性的患者有显著差异（p = 0.0002）。生存时间与性别、p53、CEA和Duke分期相关，差异均有统计学意义（p分别为0.006、0.024、0.001、0.017）。Cox回归模型显示，结直肠癌的预后受性别、p53、CEA反应性、CRC Duke分期的影响（p分别= 0.014、0.006、0.019、0.014）。总之，使用一种以上的肿瘤标志物可以成功地预测结直肠癌的预后。
Colorectal cancer (CRC) is one of the most frequent and aggressive types of cancer. Several clinicopathologic features have been studied to identify the prognostic factors that can provide information concerning the favorable or the poor outcome of colorectal cancer. In the present study, the relationship between serum CEA, p53 expression, and DNA index to the different clinicopathological characteristics of colorectal cancer patients was sought. Fifty patients with CRC were included in this study. p53 protein was detected immunohistochemically using specific monoclonal antibodies. Samples were investigated for DNA index using flow cytometry. In addition, the serum CEA was determined using ELISA. The results showed that 27/50 (54%) were positive for p53. Concerning CEA reactivity, it was found that 35/50 (70%) were reactive for CEA. These results indicate that CEA is more sensitive than p53 to detect colorectal cancer. There was a statistically significant difference between the recurrent and nonrecurrent groups in the CRC Duke's stages, survival time, serum CEA (p = 0.001, 0.016, < 0.001, respectively). Kaplan-Meier method and log-rank test showed that the mean survival time for cases positive for both p53 and CEA is significantly different from cases positive for CEA only, positive for p53 only, and negative for both p53 and CEA (p = 0.0002). Survival time was statistically significant with respect to sex, p53, CEA, and Duke's stages (p = 0.006, 0.024, 0.001, 0.017, respectively). Cox regression model showed that the prognosis of colorectal cancer is influenced by sex, p53, CEA reactivity, and CRC Duke's stages (p = 0.014, 0.006, 0.019, 0.014, respectively). In conclusion, the use of more than one tumor marker may successfully aid in the prediction of colorectal cancer prognosis.