Mitotic instability in wheat x Thinopyrum ponticum derivatives revealed by chromosome counting, nuclear DNA content and histone H3 phosphorylation pattern
Mitotic instability in wheat x Thinopyrum ponticum derivatives revealed by chromosome counting, nuclear DNA content and histone H3 phosphorylation pattern
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DOI：
10.1007/s00299-005-0913-4
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发表时间：
2005-06-01
影响因子：
6.2
通讯作者：
Guerra, M
中科院分区：
文献类型：
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作者：
Brasileiro-Vidal, AC;Brammer, S;Guerra, M
为了评价普通小麦和普通小麦衍生品(BC2F7和BC2F5加倍单倍体)的有丝分裂稳定性，采用常规和免疫染色技术进行染色体计数和DNA含量测定。对小麦父本PF-839197、轮回亲本CEP-19和对照中国春也进行了研究。经核DNA含量测定证实，杂交后代中染色体数在2n=36~60之间，以大于2n=42为优势。‘中国春’和PF 839197表现稳定，但CEP19表现出染色体数目变异(20%)。对未处理的细胞进行分析，发现存在后期桥、落后染色单体、染色体碎片和微核。用识别组蛋白H3磷酸化的抗体进行免疫染色，结果显示双着丝粒染色单体在中心染色体片段上形成后期桥和着丝粒周围的磷酸化，而在落后染色单体上没有。对观察到的有丝分裂不稳定的可能原因进行了讨论。
To evaluate the mitotic stability of Triticum aestivumxThinopyrum ponticum derivatives (BC2F7 and BC2F5 doubled haploids), chromosome counting by both conventional and immunostaining techniques, and measurement of DNA content were performed. The wheat progenitor line, PF 839197, the wheat recurrent parent CEP 19 and the control Chinese Spring were also investigated. In the hybrid derivatives, chromosome number ranged from 2n=36 to 60, with a predominance of chromosome numbers higher than 2n=42, that was confirmed by determination of nuclear DNA content. Chinese Spring' and PF 839197 were stable, but CEP 19 showed chromosome number variation (20%). Analyses of non-pretreated cells revealed the presence of anaphase bridges, lagging chromatids, chromosome fragments and micronuclei. Immunostaining with an antibody recognizing histone H3 phosphorylated showed dicentric chromatids forming anaphase bridges and pericentromeric phosphorylation at centric chromosome fragments but not at lagging chromatids. The possible causes of the observed mitotic instability are discussed.