Sex steroid receptor expression profile in brown adipose tissue. Effects of hormonal status
Sex steroid receptor expression profile in brown adipose tissue. Effects of hormonal status
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DOI：
10.1159/000110448
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Roca, Pilar
中科院分区：
文献类型：
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作者：
Rodriguez-Cuenca, Sergio;Monjo, Marta;Roca, Pilar
最近的研究表明，性激素在棕色脂肪组织（BAT）产热程序中发挥重要作用，通过作用于脂肪分解信号级联的几个步骤和UCP1转录控制。然而，关注棕色脂肪组织中类固醇受体状态的研究数量可以忽略不计。在本研究中，我们分析了雄性和雌性大鼠以及怀孕和哺乳期雌性大鼠棕色脂肪组织中类固醇受体mRNA的水平，所有这些模型都具有不同的激素背景。在体外原代培养棕色脂肪细胞中，研究了性激素对其受体表达的直接影响。雄性蝙蝠的雌激素受体（ER α）和雄激素受体（AR）密度高于雌性蝙蝠。PR A+B mRNA的表达在哺乳期下调，提示孕激素信号在这一阶段的产热损伤中起作用。体外研究表明，孕酮降低PR A+B mRNA，睾酮下调ER α mRNA。本研究强调的结果表明，在BAT和棕色细胞培养的脂肪细胞中存在类固醇受体mRNA，支持类固醇受体表达的变化对于理解性激素对BAT生理的影响很重要的观点。版权所有(c) 2007 S. Karger AG，巴塞尔。
Recent investigations suggest that sex hormones play an important role in the brown adipose tissue (BAT) thermogenic program by acting on several steps of the lipolytic signal cascade and on the UCP1 transcription control. However, the number of studies focusing on steroid receptor status in brown adipose tissue is negligible. In the present study, we analyze steroid receptor mRNA levels in brown adipose tissue in male and female rats and in pregnant and lactating females, all of them models with a different hormonal background. The direct effect of sex hormones on the expression of their receptors was studied in vitro in primary culture of brown adipocytes. Oestrogen receptor (ER alpha) and androgen receptor (AR) densities were higher in male than in female BAT. PR A+B mRNA expression was downregulated in lactation, suggesting a role of progesterone signalling in thermogenesis impairment at this stage. In vitro studies showed that progesterone decreased PR A+B mRNA and that testosterone downregulated ER alpha mRNA. The results highlighted in this study demonstrate the presence of steroid receptor mRNA in BAT and in brown cell cultured adipocytes, supporting the idea that changes in steroid receptor expression would be important for the understanding of sex hormone effects on BAT physiology. Copyright (c) 2007 S. Karger AG, Basel.