Fabrication of a modular tissue construct in a microfluidic chip
Fabrication of a modular tissue construct in a microfluidic chip
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DOI：
10.1039/b719806j
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Whitesides, George M.
中科院分区：
文献类型：
--
作者：
Bruzewicz, Derek A.;McGuigan, Alison P.;Whitesides, George M.
将微流控技术与含哺乳动物细胞凝胶的软光刻成型技术相结合，研制了一种用于哺乳动物细胞微通道三维培养的装置。细胞外基质的天然成分，包括胶原或Matrigel(TM)，构成了每一块含有细胞的凝胶的模制件(模块)的基质。每个模块在300微米以下至少有一个维度；在这些大小的模块中，进出模块的氧气、营养物质和代谢产物的流量足以使模块中的细胞增殖到与自然组织(10(8)-10(9)细胞厘米(-3))相当的密度。将模块松散地包装到微流控通道和腔室中，产生的结构渗透着一个由孔组成的网络，细胞培养液可以通过这些孔流动来喂养被包裹的细胞。随着微通道的宽度接近模块的宽度，填充组件中的顺序增加。多种细胞类型可以在空间上组织在小的微流体通道中。在恒定流量的介质(200亩L h(-1))下，24小时后对组件的回收和分析表明，在微流体室中，超过99%的包裹细胞在这一间隔中存活。
By combining microfluidics and soft-lithographic molding of gels containing mammalian cells, a device for three-dimensional (3D) culture of mammalian cells in microchannels was developed. Native components of the extracellular matrix, including collagen or Matrigel (TM), made up the matrix of each molded piece (module) of cell-containing gel. Each module had at least one dimension below similar to 300 mu m; in modules of these sizes, the flux of oxygen, nutrients, and metabolic products into and out of the modules was sufficient to allow cells in the modules to proliferate to densities comparable to those of native tissue (10(8)-10(9) cells cm(-3)). Packing modules loosely into microfluidic channels and chambers yielded structures permeated with a network of pores through which cell culture medium could flow to feed the encapsulated cells. The order in the packed assemblies increased as the width of the microchannels approached the width of the modules. Multiple cell types could be spatially organized in the small microfluidic channels. Recovery and analysis of modules after 24 h under constant flow of medium (200 mu L h(-1)) showed that over 99% of encapsulated cells survived this interval in the microfluidic chamber.