Homocysteine, glutathione and related thiols affect fertility parameters in the (sub)fertile couple
Homocysteine, glutathione and related thiols affect fertility parameters in the (sub)fertile couple
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DOI：
10.1093/humrep/del081
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发表时间：
2006-07-01
影响因子：
6.1
通讯作者：
Steegers-Theunissen, R. P. M.
中科院分区：
文献类型：
--
作者：
Ebisch, I. M. W.;Peters, W. H. M.;Steegers-Theunissen, R. P. M.
背景：巯基是活性氧（ROS）的清除剂。我们的目的是调查（亚）生育夫妇和生育结果参数的各种液体中的硫醇之间的关联。方法：在156对接受辅助生殖技术（ART）的夫妇中，我们测量了精液、纯化精子和卵泡液中谷胱甘肽（GSH）、半胱氨酸（Cys）、同型半胱氨酸（Hcy）和半胱氨酰甘氨酸（CGS）的浓度以及生育结果参数。结果：在大多数精液、精子和卵泡液中均可检测到所有巯基，其中Cys浓度最高。精液中的巯基浓度在生育力和生育力低下的男性中是相似的。子宫内膜异位症组卵泡液中Hcy水平高于特发性不孕症组（P = 0.04）。精子GSH、Cys、Hcy和CGS含量低育组显著高于特发性低育组和正常生育组（P < 0.001）。最值得注意的是，在IVF/ICSI程序中，精液和卵泡液中的Hcy浓度与培养第3天的胚胎质量呈负相关。结论：低生育力男性精子中巯基浓度明显高于生育力男性。精液和卵泡液中高同型半胱氨酸浓度对胚胎质量的不利影响是有趣的，可能表明同型半胱氨酸与生育结果呈负相关。
BACKGROUND: Thiols are scavengers of reactive oxygen species (ROS). We aim to investigate associations between thiols in various fluids in (sub)fertile couples and fertility outcome parameters. METHODS: In 156 couples undergoing assisted reproduction techniques (ART), we measured the concentrations of glutathione (GSH), cysteine (Cys), homocysteine (Hcy) and cysteinylglycine (CGS) and fertility outcome parameters in the ejaculate, purified spermatozoa and follicular fluid. RESULTS: All thiols were detectable in most ejaculates, spermatozoa and follicular fluids, of which Cys concentrations were highest. Thiol concentrations in the ejaculate were similar in fertile and subfertile men. However, Hcy in follicular fluid was higher in women with endometriosis compared with women in the idiopathic subfertile group (P = 0.04). The GSH, Cys, Hcy and CGS concentrations in spermatozoa of subfertile men were significantly higher compared with men in the idiopathic subfertile group and fertile men (P < 0.001). Most notably, Hcy concentrations in both the ejaculate and follicular fluid were negatively associated with embryo quality on culture day 3 in the IVF/ICSI procedure. CONCLUSIONS: Spermatozoa of subfertile men contain significantly higher thiol concentrations as compared with those of fertile men. The detrimental effect on embryo quality of a high Hcy concentration in the ejaculate and in follicular fluid is intriguing and may suggest that Hcy is inversely associated with fertility outcome.