Echinoid is essential for regulation of Egfr signaling and R8 formation during Drosophila eye development
Echinoid is essential for regulation of Egfr signaling and R8 formation during Drosophila eye development
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DOI：
10.1242/dev.00605
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发表时间：
2003-08
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影响因子：
--
通讯作者：
S. Spencer;R. Cagan
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文献类型：
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作者：
S. Spencer;R. Cagan
精确调节Egfr活性对正常发育和细胞分化至关重要。我们证明，在发育中的果蝇眼睛中，需要跨膜蛋白Echinoid下调Egfr活性，以确保R8感光神经元的正常排列。Echinoid是一种l1型跨膜分子，在眼视盘的所有细胞中表达，与许多其他Egfr抑制剂不同，它似乎不受转录调节。从培养细胞和眼视盘中，棘球蚴与Egfr共沉淀，Egfr活性促进棘球蚴酪氨酸磷酸化。这些观察结果表明，Echinoid通过直接相互作用抑制Egfr。
Precisely regulated Egfr activity is essential for normal development and cell differentiation. We demonstrate that the transmembrane protein Echinoid is required to downregulate Egfr activity in the developing Drosophila eye, ensuring a normal array of R8 photoreceptor neurons. Echinoid is an L1-type transmembrane molecule that is expressed in all cells of the eye imaginal discs and, unlike many other Egfr inhibitors, does not appear to be regulated transcriptionally. Echinoid co-precipitates with Egfr from cultured cells and eye imaginal discs, and Egfr activity promotes tyrosine phosphorylation of Echinoid. These observations suggest that Echinoid inhibits Egfr through direct interactions.