Quantitative analysis of postnatal neurogenesis and neuron number in the macaque monkey dentate gyrus
Quantitative analysis of postnatal neurogenesis and neuron number in the macaque monkey dentate gyrus
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DOI：
10.1111/j.1460-9568.2009.07061.x
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发表时间：
2010-01-01
影响因子：
3.4
通讯作者：
Lavenex, Pierre
中科院分区：
文献类型：
--
作者：
Jabes, Adeline;Lavenex, Pamela Banta;Lavenex, Pierre
齿状回是哺乳动物大脑中仅有的两个在出生后发生大量神经发生的区域之一。然而，有关灵长类动物齿状回出生后结构成熟的详细定量信息很少。我们进行了设计为基础的，体视学研究的神经元数量和大小，和体积的齿状回层在恒河猴（猕猴）的不同出生后年龄。我们发现，在成熟的5-10岁猕猴中观察到的颗粒细胞总数的约40%在出生后被添加到颗粒细胞层;这些神经元中的25%在出生后的前三个月内被添加。因此，齿状回内的细胞增殖和神经发生在出生后的前3个月内达到峰值，并在3个月和至少1岁之间保持在中间水平。虽然颗粒细胞体在生命的第一年经历了最大的增长，细胞的大小和体积的三层齿状回（即分子，颗粒细胞和多态层）继续增加超过1岁。此外，不同层次的齿状回表现出不同的体积变化在出生后的发展。最后，我们观察到显着水平的细胞增殖，神经发生和细胞死亡的背景下，一个整体稳定数量的颗粒细胞在成熟的5-10岁的猴子。这些数据确定了一个延长的发育期，在此期间，神经发生可能被调制，以显着影响成年期齿状回的结构和功能。
The dentate gyrus is one of only two regions of the mammalian brain where substantial neurogenesis occurs postnatally. However, detailed quantitative information about the postnatal structural maturation of the primate dentate gyrus is meager. We performed design-based, stereological studies of neuron number and size, and volume of the dentate gyrus layers in rhesus macaque monkeys (Macaca mulatta) of different postnatal ages. We found that about 40% of the total number of granule cells observed in mature 5-10-year-old macaque monkeys are added to the granule cell layer postnatally; 25% of these neurons are added within the first three postnatal months. Accordingly, cell proliferation and neurogenesis within the dentate gyrus peak within the first 3 months after birth and remain at an intermediate level between 3 months and at least 1 year of age. Although granule cell bodies undergo their largest increase in size during the first year of life, cell size and the volume of the three layers of the dentate gyrus (i.e. the molecular, granule cell and polymorphic layers) continue to increase beyond 1 year of age. Moreover, the different layers of the dentate gyrus exhibit distinct volumetric changes during postnatal development. Finally, we observe significant levels of cell proliferation, neurogenesis and cell death in the context of an overall stable number of granule cells in mature 5-10-year-old monkeys. These data identify an extended developmental period during which neurogenesis might be modulated to significantly impact the structure and function of the dentate gyrus in adulthood.