Discrimination of closely homologous human genomic and viral sequences in cells and tissues: further characterization of Tmt
Discrimination of closely homologous human genomic and viral sequences in cells and tissues: further characterization of Tmt
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细胞和组织中紧密同源的人类基因组和病毒序列的区分：Tmt 的进一步表征
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
J. O'D. McGEE
中科院分区：
文献类型：
--
作者：
C. Herrington;J. O'D. McGEE
SummaryThe experimentally derived parameter Tmt (tissue Tm) was defined previously to describe the end-point used for evaluation of the stringency of non-isotopic in situ hybridization and was found to differ from the theoretical melting temperature (Tm) for several HPV types. In this paper, the reasons for this discrepancy were investigated by performing a series of experiments with a variety of probes for both human genomic and integrated viral sequences in isolated and cultured normal and abnormal cells in addition to paraffin-embedded material. Tmt was shown to be dependent on several parameters of probe and target, and on the sensitivity of the detection system used but was not affected by aldehyde fixation or paraffin wax embedding under optimal conditions of nucleic acid unmasking. These data support the hypothesis that differences between Tmt and Tm may be due to the use of a different end-point for in situ hybridization analysis rather than biochemical alteration of DNA-DNA interactions in intact cells. Appropriate stringency conditions should therefore be determined by experiment rather than calculated theoretically for gene evaluation in cells and tissues.
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC
来自人类 1 号染色体的染色体特异性 α 卫星 DNA：跨越数百千碱基对着丝粒 DNA 的多态性结构域的层次结构和基因组组织。
影响因子：
4.4
作者：
Waye,JS;Durfy,SJ;Pinkel,D;Kenwrick,S;Patterson,M;Davies,KE;Willard,HF
通讯作者：
Willard,HF