Exploration of rice pistil responses during early post-pollination through a combined proteomic and transcriptomic analysis
Exploration of rice pistil responses during early post-pollination through a combined proteomic and transcriptomic analysis
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DOI：
10.1016/j.jprot.2015.11.004
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发表时间：
2016-01-10
影响因子：
3.3
通讯作者：
Yang, Pingfang
中科院分区：
文献类型：
--
作者：
Li, Ming;Wang, Kun;Yang, Pingfang
花粉与柱头的相互作用是一个多步骤的复杂生理过程，包含着不同的信号转导和生化途径。然而，很少有人知道这一过程在水稻（Oryza sativa）的分子机制。本研究采用转录组和蛋白质组相结合的方法，研究水稻雌蕊授粉过程中基因表达的变化。共鉴定出1117个差异表达基因，其中962个在转录水平上表达，167个在蛋白水平上表达。功能分类分析表明，参与中枢代谢的基因表达上调，可能导致这些代谢的增强。活性氧（ROS）在柱头中过量积累。作为对这一点的响应，参与氧化还原稳态调节的蛋白质或转录物差异表达。此外，蛋白质泛素化及其相关基因或蛋白质，特别是一些E3连接酶编码基因的显著变化表明，蛋白质泛素化可能在授粉过程中的细胞信号转导中发挥重要作用。本研究揭示了水稻雌蕊授粉过程中基因和蛋白质的表达谱，为水稻授粉调控的分子机制提供了一个全面的认识。生物学意义：利用RNA-seq、2-DE和iTRAQ技术，获得了大量的转录组和蛋白质组数据，其中包含了丰富的花粉和雌蕊相互作用相关基因的信息。结果表明，授粉后柱头中活性氧显著积累，氧化还原稳态系统相关基因的丰度发生了不同程度的变化。我们还发现，一些E3连接酶编码基因的变化可能表明蛋白质泛素化在授粉过程中的细胞信号转导中起重要作用。本研究的数据有助于深入了解水稻的授粉过程。(C)2015 Elsevier B. V.版权所有。
Pollen-stigma interaction is a multi-step and complex physiological process which contains different signaling and biochemical pathways. However, little is known about the molecular mechanism underlying this process in rice (Oryza saliva). In this study, the changes of gene expression were investigated through a combination study of transcriptome and proteome profiles in rice pistil during pollination. Totally, 1117 differentially expressed genes were identified, among which 962 and 167 were detected at transcriptional and protein level respectively. Functional categorization analysis showed that the genes involved in central metabolism were up-regulated, which can lead to the enhancement of these metabolisms. The reactive oxygen species (ROS) were over-accumulated in the stigma. In response to this, the proteins or transcripts involved in redox homeostasis regulation were differentially expressed. Furthermore, significant changes of protein ubiquitination and its related genes or proteins, especially some E3 ligases encoding genes, indicate that protein ubiquitination might play important roles in cell signal transduction during the pollination process. Our study sheds some lights on gene and protein expression profiles of rice pistil pollination process, and gives us a comprehensive understanding of the basic molecular mechanisms controlling pollination in rice.Biological significance: Using RNA-seq, 2-DE and iTRAQ assays, we have generated the large-scale transcriptomic and proteomic data containing abundant information on genes involved in pollen and pistil interaction. Our results showed that ROS were significantly accumulated in stigma after pollination, and the abundance of genes involve in redox homeostasis system were changed variously. We also show that, changes of some E3 ligases encoding genes might indicate that protein ubiquitination play important roles in cell signal transduction during the pollination process. Data in this study might be helpful to deeply understand the pollination in rice. (C) 2015 Elsevier B.V. All rights reserved.