Hsp90 is involved in the regulation of cytosolic precursor protein abundance in tomato.
Hsp90 is involved in the regulation of cytosolic precursor protein abundance in tomato.
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DOI：
10.1016/j.molp.2014.10.005
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发表时间：
2015-02
期刊：
影响因子：
27.5
通讯作者：
Bodo Tillmann;Sascha Röth;D. Bublak;M. Sommer;E. Stelzer;K. Scharf;E. Schleiff
中科院分区：
文献类型：
--
作者：
Bodo Tillmann;Sascha Röth;D. Bublak;M. Sommer;E. Stelzer;K. Scharf;E. Schleiff
胞质分子伴侣一般参与细胞蛋白质稳态的调节。热应激蛋白70（Hsp 70）和90（Hsp 90）家族的成员帮助前蛋白转运到细胞器，如叶绿体或线粒体。此外，热休克蛋白70被描述为参与降解积累在细胞质中的叶绿体前蛋白。由于Hsp 90没有建立类似的功能，我们分析了Hsp 90和Hsp 70对蛋白质丰度的影响，在细胞环境中使用基于叶肉原生质体的体内系统。我们观察到的差异行为的前蛋白质的胞质伴侣依赖性的调节。一些前蛋白如pOE 33显示出对Hsp 90的高度依赖性，而前蛋白如pSSU的丰度更强烈地依赖于Hsp 70。E3连接酶，热休克蛋白70相互作用蛋白（芯片）的C-末端，似乎有一个更普遍的作用，在胞质蛋白丰度的控制。我们讨论了为什么不同的反应模式是可比的胞质未折叠蛋白质的反应。
Cytosolic chaperones are involved in the regulation of cellular protein homeostasis in general. Members of the families of heat stress proteins 70 (Hsp70) and 90 (Hsp90) assist the transport of preproteins to organelles such as chloroplasts or mitochondria. In addition, Hsp70 was described to be involved in the degradation of chloroplast preproteins that accumulate in the cytosol. Because a similar function has not been established for Hsp90, we analyzed the influences of Hsp90 and Hsp70 on the protein abundance in the cellular context using anin vivosystem based on mesophyll protoplasts. We observed a differential behavior of preproteins with respect to the cytosolic chaperone-dependent regulation. Some preproteins such as pOE33 show a high dependence on Hsp90, whereas the abundance of preproteins such as pSSU is more strongly dependent on Hsp70. The E3 ligase, C-terminus of Hsp70-interacting protein (Chip), appears to have a more general role in the control of cytosolic protein abundance. We discuss why the different reaction modes are comparable with the cytosolic unfolded protein response.