Whole-cell recording in isolated primary sensory neurons.
Whole-cell recording in isolated primary sensory neurons.
复制标题
分离的初级感觉神经元的全细胞记录。
DOI：
10.1007/978-1-61779-561-9_5
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Gold,MichaelS
中科院分区：
文献类型：
--
作者：
Gold,MichaelS
体外分离的感觉神经元是一个强有力的模型，它可以解决许多重要的神经生物学问题。分离的神经元相对容易从新生和成年动物中制备，并且可以在急性和培养相当长时间后进行研究。细胞内记录是研究这些神经元的最有力的方法之一。方法被描述为两个分离的感觉神经元在体外的准备，以及记录主要类别的离子电流（Na+，K+，和Ca 2+）从这些神经元与全细胞电压钳技术。还提供了用于在电流钳中对这些神经元的主动和被动电生理特性进行初始表征的方法，以及使用穿孔膜片记录作为减轻与常规全细胞膜片记录相关联的一些限制的手段。读者应该知道，感觉神经元中离子通道的调节可能非常微妙，需要比这里提供的复杂得多的协议。读者还应该意识到，感觉神经元之间存在巨大的异质性，这对那些希望研究它们的人来说既是诅咒又是祝福。因此，这里提供的方法应该仅仅被认为是对感觉神经元进行更详细分析的起点。
The isolated sensory neuron in vitro is a powerful model with which to address a number of important neurobiological questions. Isolated neurons are relatively easy to prepare from both neonatal and adult animals and can be studied both acutely and after considerable time on culture. Intracellular recording is one of the most powerful ways to study these neurons. Methods are described for both the preparation of isolated sensory neurons in vitro as well as for recording major classes of ionic currents (Na+, K+, and Ca2+) from these neurons with whole cell voltage-clamp techniques. Methods are also provided for an initial characterization of active and passive electrophysiological properties of these neurons in current clamp as well as the use of perforated patch recording as a means to mitigate some of the limitations associated with conventional whole cell patch recording. The reader should be aware that the regulation of ion channels in sensory neurons may very subtle, requiring considerably more sophisticated protocols than have been provided here. The reader should also be aware that there is a tremendous heterogeneity among sensory neurons, which is both a curse and a blessing for those who wish to study them. Thus, the methods provided here should only be considered the starting point for a more detailed analysis of sensory neurons.
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DOI：
10.1016/s0169-328x(02)00411-4
发表时间：
2002-10-15
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Renganathan, M;Dib-Hajj, S;Waxman, SG
通讯作者：
Waxman, SG
影响因子：
2.5
作者：
SCROGGS, RS;TODOROVIC, SM;FOX, AP
通讯作者：
FOX, AP
影响因子：
2.5
作者：
Gold, MS;Shuster, MJ;Levine, JD
通讯作者：
Levine, JD
影响因子：
2.5
作者：
Harriott, Andrea M.;Gold, Michael S.
通讯作者：
Gold, Michael S.
影响因子：
2.5
作者：
CARDENAS, CG;DELMAR, LP;SCROGGS, RS
通讯作者：
SCROGGS, RS