Regulation of ACTH Secretion from Corticotrophs: The Interaction of Vasopressin and CRF a
Regulation of ACTH Secretion from Corticotrophs: The Interaction of Vasopressin and CRF a
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促肾上腺皮质激素分泌 ACTH 的调节：加压素和 CRF 的相互作用
DOI：
--
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发表时间：
1987
影响因子：
5.2
通讯作者：
W. Vale
中科院分区：
文献类型：
--
作者：
L. Bilezikjian;W. Vale
ACTH的分泌受下丘脑合成和分泌的多种激素调节。主要的控制因素是促肾上腺皮质激素释放因子(CRF)，它对ACTH既有分泌又有合成作用。后者包括加压素(AVP)、催产素、血管紧张素I1和CAT ECHO。外周来源的糖皮质激素对ACTH的分泌和合成都起负反馈控制作用。在培养的大鼠垂体前叶细胞和垂体前叶细胞中都证实了C R F的特异性高亲和力受体。现有数据表明C R F的作用是通过cAMP依赖的途径介导的。这种激素刺激完整细胞中cAMP的生成16*1‘-20和与垂体前质膜相关的腺苷环化酶活性。在克隆的ACTH分泌细胞系中，C R F被证明刺激cAMP依赖的蛋白激酶。22另一方面，AVP的作用还没有得到很好的描述。垂体前叶血管加压素受体的特性与文献报道的相似，后者通过促进磷脂酶C催化的磷脂酰肌醇二磷酸(PIP_2)促进磷脂酰肌醇(PI)的周转来促进磷脂酰肌醇(PI)的代谢。此外，在培养的大鼠垂体前叶细胞中，AVP对~(32)P的刺激和PI的标记也提示了这种激素在两种组织中的共同作用机制。PIP2的水解导致细胞内产生双重信号，三磷酸肌醇(IP3)和二酰甘油(DAG)，分别升高细胞内钙离子和激活蛋白激酶C(PKC)。外源添加佛波酯(如佛波醇肉豆蔻酸酯(TPA)和合成DAG(如二辛酰甘油(DiC8))可直接激活PKC。33这些试剂也对ACTH的分泌有刺激作用。34s35因此，它们被用作工具来确定AVP对皮质细胞的作用是否可能是通过PLC催化的PI P2水解途径来介导的。在单个细胞类型中存在多个细胞内第二信使通路可以提供使细胞能够对输入信号做出适当反应的“安全”机制。尽管在现有信令中可能存在一些重叠
The secretion of ACTH is multihormonally regulated by factors synthesized and secreted by the hypothalamus. The primary control is mediated by corticotropin releasing factor (CRF), which has both secretory and synthetic effects on ACTH.'-9 Other factors are either weak secretagogues or have facilitatory actions on CRFmediated secretion. Among the latter are vasopressin (AVP), oxytocin, angiotensin I1 and the cat echo la mine^.^*^ I n addition, glucocorticoids of peripheral origin exert a negative feedback control on both ACTH secretion and synthe~is .~.~* '" Specific high-affinity receptors for C R F have been demonstrated both in cultured rat anterior pituitary cells and anterior pituitary h~mogenates."-'~ Available data suggest that the actions of C R F are mediated by the CAMP-dependent pathway.'"20 This hormone stimulates CAMP generation in intact cells16*1'-20 and adenylate cyclase activity associated with anterior pituitary plasma membrane^.'^.'^ In a clonal ACTHsecreting cell line, the AtT20 cells, C R F was shown to stimulate CAMP-dependent protein kinases,21 and the insertion of the inhibitor of this protein kinase inhibited ACTH secretion and POMC gene expression.22 The action of AVP, on the other hand, has not been so well characterized. Anterior pituitary receptors for AVP appear to have characteristics similar to those found in h e p a t o c y t e ~ ~ ~ ~ ~ where AVP stimulates phosphatidylinositol (PI) turnover by enhancing phospholipase C-catalyzed phosphatidylinositol bisphosphate (PIP2) h y d r o l y ~ i s . ~ ~ Furthermore, the stimulation of 32P, labeling of PI in response to AVP in cultured rat anterior pituitary cells2' suggests a common mechanism for this hormone in both tissues. The hydrolysis of PIP2 results in the generation of dual intracellular signals, inositol trisphosphate (IP3) and diacylglycerol (DAG), which elevate intracellular Ca2+ and activate protein kinase C (PKC), re~pect ively.~~-~* Exogenously added phorbol esters, such as phorbol myristate acetate (TPA), and synthetic DAG, such as dioctanoylglycerol (DiC8), activate PKC directly.33 These agents also have stimulatory effects on ACTH secretion.34s35 Therefore, they have been used as tools to determine if the action of AVP on corticotrophs might be mediated by the PLCcatalyzed PI P2 hydrolysis pathway. The existence of multiple intracellular second messenger pathways in a single cell type may provide a "safety" mechanism that enables the cells to respond appropriately to incoming signals. Although there may be some overlap in the existing signaling
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DOI：
10.1073/pnas.78.10.6517
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SPIESS, J;RIVIER, J;VALE, W
通讯作者：
VALE, W
AtT20 细胞中 cAMP 依赖性蛋白激酶的激素激活。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Litvin,Y;PasMantier,R;Fleischer,N;Erlichman,J
通讯作者：
Erlichman,J
使用 CRF 的荧光类似物鉴定促肾上腺皮质激素释放因子 (CRF) 靶细胞以及地塞米松对垂体前叶结合的影响。
影响因子：
4.8
作者：
Schwartz,J;Billestrup,N;Perrin,M;Rivier,J;Vale,W
通讯作者：
Vale,W
影响因子：
4.8
作者：
M. Perrin;Yaira Haas;J. Rivier;W. Vale
通讯作者：
M. Perrin;Yaira Haas;J. Rivier;W. Vale
合成绵羊促肾上腺皮质激素释放因子、糖皮质激素、儿茶酚胺、神经垂体肽和其他物质对培养促肾上腺皮质激素细胞的影响。
影响因子：
4.8
作者：
Vale,W;Vaughan,J;Smith,M;Yamamoto,G;Rivier,J;Rivier,C
通讯作者：
Rivier,C