Measurement of equine myeloperoxidase (MPO) activity in synovial fluid by a modified MPO assay and evaluation of joint diseases - An initial case study
Measurement of equine myeloperoxidase (MPO) activity in synovial fluid by a modified MPO assay and evaluation of joint diseases - An initial case study
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DOI：
10.1016/j.rvsc.2007.06.006
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发表时间：
2008-06-01
影响因子：
2.4
通讯作者：
Einspanier, R.
中科院分区：
文献类型：
--
作者：
Fietz, S.;Bondzio, A.;Einspanier, R.
本研究的目的是开发一种特定的髓过氧化物酶（MPO）活性测定在滑液的马，并调查是否MPO活性增加，在不同形式的关节疾病。从患有骨关节炎、慢性非脓毒性关节炎和脓毒性关节炎的马以及健康对照马的受影响关节采集滑液样品。使用含有4-氨基苯甲酸酰肼作为MPO的有效和特异性抑制剂的特异性改良邻联茴香胺测定法测量MPO活性。该测定的特征在于高重现性。结果显示，骨关节炎和慢性非化脓性关节炎的马的滑液中MPO活性仅轻微升高。然而，在脓毒性关节炎的情况下，MPO活性显着增加时，发现与对照组相比。总之，第一个领域的研究表明，滑液髓过氧化物酶可作为一个标志物的化脓性关节炎的马。(C)2007爱思唯尔有限公司保留所有权利。
The aim of this study was to develop a specific myeloperoxidase (MPO) activity assay in the synovial fluid of horses and investigate whether MPO activity is increased in different forms of joint diseases. Synovial fluid samples were taken from affected joints from horses with osteoarthritis, chronic non-septic arthritis and septic arthritis, and from healthy control horses. MPO activity was measured using a specific modified o-dianisidine-assay containing 4-aminobenzoic acid hydrazide as a potent and specific inhibitor of the MPO. This assay is characterized by high reproducibility. The results reveal only a slight elevation of MPO activity in the synovial fluid of horses with osteoarthritis and chronic non-septic arthritis. However, in the cases of septic arthritis a significant increase in MPO activity was found when compared to the controls. In conclusion the first field study suggests that synovial fluid MPO may be used as a marker for septic arthritis in horses. (C) 2007 Elsevier Ltd. All rights reserved.