The Molecular Structure of Human Red Blood Cell Membranes from Highly Oriented, Solid Supported Multi-Lamellar Membranes
The Molecular Structure of Human Red Blood Cell Membranes from Highly Oriented, Solid Supported Multi-Lamellar Membranes
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DOI：
10.1038/srep39661
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发表时间：
2017-01-03
影响因子：
4.6
通讯作者：
Rheinstadter, Maikel C.
中科院分区：
文献类型：
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作者：
Himbert, Sebastian;Alsop, Richard J.;Rheinstadter, Maikel C.
我们在硅片上制备了高度定向、多层堆叠的人红细胞(RBC)膜。通过溶血法制备了RBC膜，并将其应用于功能化硅片上，然后将其退火成多层红细胞膜。用高分辨X射线衍射仪测定了堆叠膜的分子结构。我们提供了直接的实验证据，这些红细胞膜由完整的卷曲多肽的纳米结构域组成，以及液体有序(L(O))和液体无序(L(D))脂类。确定了薄层间距、膜和水合水层厚度、每脂尾面积和结构域大小。将常用药物阿司匹林添加到红细胞膜中，发现其与红细胞膜相互作用，并且优选地在L(O)结构域的头部基团区域中分配，导致膜的流态化，即双层变薄和脂尾间距增加。我们的结果进一步支持了当前红细胞膜为片状结构的模型，并提供了不同领域中分子组织的前所未有的结构细节。
We prepared highly oriented, multi-lamellar stacks of human red blood cell (RBC) membranes applied on silicon wafers. RBC ghosts were prepared by hemolysis and applied onto functionalized silicon chips and annealed into multi-lamellar RBC membranes. High resolution X-ray diffraction was used to determine the molecular structure of the stacked membranes. We present direct experimental evidence that these RBC membranes consist of nanometer sized domains of integral coiled-coil peptides, as well as liquid ordered (l(o)) and liquid disordered (l(d)) lipids. Lamellar spacings, membrane and hydration water layer thicknesses, areas per lipid tail and domain sizes were determined. The common drug aspirin was added to the RBC membranes and found to interact with RBC membranes and preferably partition in the head group region of the l(o) domain leading to a fluidification of the membranes, i.e., a thinning of the bilayers and an increase in lipid tail spacing. Our results further support current models of RBC membranes as patchy structures and provide unprecedented structural details of the molecular organization in the different domains.