Electrophysiological characterization of rat and mouse olfactory receptor neurons from an intact epithelial preparation
Electrophysiological characterization of rat and mouse olfactory receptor neurons from an intact epithelial preparation
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DOI：
10.1016/s0165-0270(99)00089-8
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发表时间：
1999-10-15
影响因子：
3
通讯作者：
Shepherd, GM
中科院分区：
文献类型：
--
作者：
Ma, MH;Chen, WR;Shepherd, GM
为了了解嗅觉识别的编码机制，有必要对嗅觉感受器神经元的气味反应特性进行表征。与分子生物学的快速发展形成鲜明对比的是，有关啮齿类动物的Orns的生理学数据很少。为了便于获取这些数据，我们开发了一种来自大鼠和小鼠的完整的嗅觉上皮制剂。我们通过膜片钳直接在ORN树突状结节上监测气味反应，对这种制剂进行了初步研究。ORN树突状结节是一个非常靠近嗅觉信号转导轨迹的亚细胞位置。我们的结果表明，大鼠和小鼠的卵母细胞具有相似的固有膜特性。大多数细胞自发地以低频率(F)激发，大约一半的细胞响应电流(I)注入以线性f/i关系重复激发。大约三分之一的大鼠和小鼠对四种气味的混合物有剂量依赖的反应，其中大约60%的大鼠和小鼠对IBMX有反应，IBMX是一种有效的磷酸二酯酶抑制剂。结果表明，这种完整的制备方法提供了接近体内生理条件的优势，同时提供了使用电生理或细胞成像方法以空间定义的方式映射上皮中单个神经元反应的机会。(C)1999 Elsevier Science B.V.保留所有权利。
To understand the coding mechanisms underlying olfactory discrimination, it is necessary to characterize odor response properties of olfactory receptor neurons (ORNs). In contrast with rapid progress in molecular biology, there is little physiological data from ORNs in rodent. To facilitate acquisition of such data, we have developed an intact olfactory epithelial preparation from both rat and mouse. We have carried out initial studies of this preparation by monitoring odor responses by patch-clamping directly on the ORN dendritic knobs, a subcellular site very close to the locus of olfactory signal transduction. Our results show that rat and mouse ORNs have similar intrinsic membrane properties. Most cells fired spontaneously at a low frequency (f) and about one half fired repetitively in response to current (I) injection with a linear f/I relation. About one third of rat and mouse ORNs responded to a mixture of four odors in a dose-dependent manner and about 60% of them responded to IBMX, a potent inhibitor of phosphodiesterase. The results suggest that this intact preparation offers the advantage of approximating in vivo physiological conditions, while furnishing an opportunity to map single neuron responses in the epithelium in a spatially-defined manner, using electrophysiological or cell imaging methods. (C) 1999 Elsevier Science B.V. All rights reserved.