Virucidal efficacy of hydrogen peroxide vapour disinfection
Virucidal efficacy of hydrogen peroxide vapour disinfection
复制标题
DOI：
10.1016/j.jhin.2011.10.012
复制
发表时间：
2012-02-01
影响因子：
6.9
通讯作者：
Duizer, E.
中科院分区：
文献类型：
--
作者：
Tuladhar, E.;Terpstra, P.;Duizer, E.
背景：表面的病毒污染被认为是传播的重要因素。化学消毒可以是一种有效的干预手段，但鲜为人知的是，过氧化氢蒸汽（HPV）对肠道和呼吸道virus.Aim的杀病毒效果：衡量HPV对呼吸道和肠道病毒的杀病毒效果的材料上代表那些发现在institutions and homes.Methods：脊髓灰质炎病毒，人类诺如病毒基因组II。4（GII. 4），将干燥到不锈钢上的鼠诺如病毒1、轮状病毒、腺病毒和流感A（H1N1）病毒、框架板和纱布载体在室温下在隔离器中暴露于HPV 127 ppm 1小时。脊髓灰质炎病毒也在房间的不同位置暴露于HPV。通过比较可回收病毒滴度与未暴露对照品，测定杀病毒效果。聚合酶链反应用于评估HPV对病毒基因组减少的影响。HPV消毒导致所有受试病毒完全灭活，其特征在于不锈钢和框架板载体上脊髓灰质炎病毒、轮状病毒、腺病毒和鼠诺如病毒的感染性颗粒减少>4 log（10），不锈钢和框架板载体上甲型流感病毒减少>2 log（10），以及纱布载体上的所有病毒。在房间的几个位置证明脊髓灰质炎病毒完全灭活。在框架板和纱布载体上病毒基因组的减少是最小的，但在不锈钢载体上是显著的;人诺如病毒GII。结论：HPV是一种有效的杀灭肠道和呼吸道病毒的药物。(C)2011年，卫生保健感染协会。由爱思唯尔有限公司出版。保留所有权利。
Background: Viral contamination of surfaces is thought to be important in transmission. Chemical disinfection can be an effective means of intervention, but little is known about the virucidal efficacy of hydrogen peroxide vapour (HPV) against enteric and respiratory viruses.Aim: To measure the virucidal efficacy of HPV against respiratory and enteric viruses on materials representing those found in institutions and homes.Methods: Poliovirus, human norovirus genogroup II. 4 (GII. 4), murine norovirus 1, rotavirus, adenovirus and influenza A (H1N1) virus dried on to stainless steel, framing panel and gauze carriers were exposed to HPV 127 ppm for 1 h at room temperature in an isolator. Poliovirus was also exposed to HPV at different locations in a room. The virucidal effect was measured by comparing recoverable viral titres against unexposed controls. Polymerase chain reaction was used to evaluate the effect of HPV on viral genome reduction.Findings: HPV disinfection resulted in complete inactivation of all viruses tested, characterized by >4 log(10) reduction in infectious particles for poliovirus, rotavirus, adenovirus and murine norovirus on stainless steel and framing panel carriers, and >2 log(10) reduction for influenza A virus on stainless steel and framing panel carriers, and for all viruses on gauze carriers. Complete inactivation of poliovirus was demonstrated at several locations in the room. Reductions in viral genomes were minimal on framing panel and gauze carriers but significant on stainless steel carriers; human norovirus GII. 4 genome was most resistant to HPV treatment.Conclusion: HPV could be an effective virucidal against enteric and respiratory viruses contaminating in-house environments. (C) 2011 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.