The developmental transcriptome of Drosophila melanogaster.
The developmental transcriptome of Drosophila melanogaster.
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DOI：
10.1038/nature09715
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发表时间：
2011-03-24
期刊：
影响因子：
64.8
通讯作者：
Celniker SE
中科院分区：
文献类型：
--
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
黑腹果蝇是研究最为深入的遗传模式生物之一，然而其基因组仍然包含未注释的编码基因和非编码基因、转录本、外显子以及RNA编辑位点。全面的发现和注释是理解转录、剪接和RNA编辑的调控如何指导这种复杂生物发育的先决条件。我们利用RNA测序、芯片测序以及cDNA测序来探索30个不同发育阶段的转录组。我们鉴定出111,195个新元件，包括数千个基因、编码和非编码转录本、外显子、剪接和编辑事件，并推断出先前使用已有的实验、预测以及基于保守性的方法未能发现的蛋白质异构体。总之，这些数据极大地扩充了果蝇基因组中已知转录元件的数量，并提供了整个发育过程中转录组动态的高分辨率视图。
Drosophila melanogaster is one of the most well studied genetic model organisms, nonetheless its genome still contains unannotated coding and non-coding genes, transcripts, exons, and RNA editing sites. Full discovery and annotation are prerequisites for understanding how the regulation of transcription, splicing, and RNA editing directs development of this complex organism. We used RNA-Seq, tiling microarrays, and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. Together, these data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.