Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo
Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo
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DOI：
10.4012/dmj.23.650
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发表时间：
2004-12-01
影响因子：
2.5
通讯作者：
Takahashi, J
中科院分区：
文献类型：
--
作者：
Hamada, Y;Yuki, K;Takahashi, J
我们之前的研究报道了骨桥蛋白衍生肽SVVYGLR在体外激活内皮细胞的粘附、迁移和管形成能力。本研究调查了体内合成 SVVYGLR 和突变肽引起的血管生成。通过用丙氨酸 (A) 取代构成 SVVYGLR 的 7 个氨基酸之一来合成突变肽 (n = 7)。在背气囊测定中，植入充满 SVVYGLR 的腔室 5 天后，小鼠背部皮肤的新形成血管数量与充满血管内皮生长因子 (VEGF) 的腔室数量大致相同。 SVVAGLR的血管生成能力显着低于其他6种突变肽和SVVYGLR。这表明酪氨酸(Y)在SVVYGLR引起的血管生成中发挥重要作用。
Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.