HTLV-I and HTLV-II tax trans-activate the human EGR-1 promoter through different cis-acting sequences.
HTLV-I and HTLV-II tax trans-activate the human EGR-1 promoter through different cis-acting sequences.
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发表时间：
1992-11
期刊：
影响因子：
8
通讯作者：
K. Sakamoto;S. Nimer;J. Rosenblatt;J. Gasson
中科院分区：
文献类型：
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作者：
K. Sakamoto;S. Nimer;J. Rosenblatt;J. Gasson
即早反应基因EGR-1编码一种含锌指的转录因子，参与多种细胞类型的生长和分化。EGR-1在促有丝分裂刺激后在正常T细胞中被诱导，并且最近已显示在人T细胞白血病病毒I型（HTLV-I）和II型（HTLV-II）转化的T细胞系中组成型表达。HTLV-I的反式激活蛋白Tax已被证明可以反式激活许多细胞基因的启动子，其中一些可能在调节T细胞增殖中至关重要。在这项研究中，我们研究的EGR-1在三个T细胞系的表达税的影响，并证明HTLV-I和-II税能够反式激活人EGR-1重组启动子结构。有趣的是，HTLV-I和-II Tax通过Jurkat细胞系中不同的启动子区域反式激活人EGR-1启动子，表明HTLV-I和-II Tax可能通过不同的信号传导途径导致EGR-1的组成型表达。EGR-1的表达失调可能有助于在HTLV-1和-II感染的细胞中T细胞活化的早期阶段不受控制的细胞生长和转化。
The immediate-early response gene, EGR-1, encodes a zinc finger-containing transcription factor that is involved in growth and differentiation of a variety of cell types. EGR-1 is induced in normal T cells following mitogenic stimulation and has recently been shown to be constitutively expressed in human T-cell leukemia virus type I (HTLV-I)- and type II (HTLV-II)-transformed T-cell lines. The trans-activating protein of HTLV-I, Tax, has been demonstrated to trans-activate promoters of a number of cellular genes, some of which may be critical in regulating T-cell proliferation. In this study, we examine the effect of Tax on expression of EGR-1 in three T-cell lines and demonstrate that both HTLV-I and -II Tax are capable of trans-activating human EGR-1 recombinant promoter constructs. Interestingly, HTLV-I and -II Tax trans-activate the human EGR-1 promoter through different promoter regions in the Jurkat cell line, suggesting that HTLV-I and -II Tax may lead to constitutive expression of EGR-1 through different signaling pathways. Deregulated expression of EGR-1 may contribute to uncontrolled cell growth and transformation during early stages of T-cell activation in HTLV-I and -II-infected cells.