Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses
Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses
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DOI：
10.1105/tpc.010410
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发表时间：
2002-03-01
期刊：
影响因子：
11.6
通讯作者：
Zhu, T
中科院分区：
文献类型：
--
作者：
Chen, WQ;Provart, NJ;Zhu, T
大量研究表明，转录因子在调节植物对环境胁迫的反应中起着重要作用。然而，大多数编码转录因子的基因的具体功能尚不清楚。在这项研究中，我们使用基因芯片实验产生的mRNA谱推断已知和推定的拟南芥转录因子编码基因的功能。402个不同的转录因子基因的mRNA水平在不同的发育阶段和不同的胁迫条件下进行了检查。通过观察某些胁迫处理后基因的激活和抑制，鉴定了在胁迫信号转导途径中潜在控制下游基因表达的转录因子。一些先前表征的转录因子基因的mRNA水平在与其他调控途径的连接中发生了显着变化，表明其多功能性。响应细菌病原体感染的74个转录因子基因的表达在水杨酸、茉莉酸或乙烯信号传导缺陷的突变体中减少或消除。这一观察结果表明，这些基因的调节至少部分是由这些植物激素介导的，并表明转录因子基因参与调节这些激素介导的额外的下游反应。在衰老过程中诱导的43个转录因子基因中，有28个也被胁迫诱导，表明对这些胁迫的广泛重叠反应。对冷胁迫基因启动子区的统计分析表明，已知的保守的转录因子结合位点的响应明确的富集。一个高度保守的新的启动子基序被确定在基因中响应一个广泛的病原体感染治疗。这一观察结果有力地表明，相应的转录因子在这些特定调节子的协调调节中起着普遍和关键的作用。虽然需要进一步验证，这些相关的结果提供了大量的信息，可以指导假说驱动的研究，以阐明参与转录调控和信号网络在植物中的分子机制。
Numerous studies have shown that transcription factors are important in regulating plant responses to environmental stress. However, specific functions for most of the genes encoding transcription factors are unclear. In this study, we used mRNA profiles generated from microarray experiments to deduce the functions of genes encoding known and putative Arabidopsis transcription factors. The mRNA levels of 402 distinct transcription factor genes were examined at different developmental stages and under various stress conditions. Transcription factors potentially controlling downstream gene expression in stress signal transduction pathways were identified by observed activation and repression of the genes after certain stress treatments. The mRNA levels of a number of previously characterized transcription factor genes were changed significantly in connection with other regulatory pathways, suggesting their multifunctional nature. The expression of 74 transcription factor genes responsive to bacterial pathogen infection was reduced or abolished in mutants that have defects in salicylic acid, jasmonic acid, or ethylene signaling. This observation indicates that the regulation of these genes is mediated at least partly by these plant hormones and suggests that the transcription factor genes are involved in the regulation of additional downstream responses mediated by these hormones. Among the 43 transcription factor genes that are induced during senescence, 28 of them also are induced by stress treatment, suggesting extensive overlap responses to these stresses. Statistical analysis of the promoter regions of the genes responsive to cold stress indicated unambiguous enrichment of known conserved transcription factor binding sites for the responses. A highly conserved novel promoter motif was identified in genes responding to a broad set of pathogen infection treatments. This observation strongly suggests that the corresponding transcription factors play general and crucial roles in the coordinated regulation of these specific regulons. Although further validation is needed, these correlative results provide a vast amount of information that can guide hypothesis-driven research to elucidate the molecular mechanisms involved in transcriptional regulation and signaling networks in plants.