An integrated microspectrometer for localised multiplexing measurements.
An integrated microspectrometer for localised multiplexing measurements.
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DOI：
10.1039/c4lc00952e
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发表时间：
2015-01
期刊：
影响因子：
6.1
通讯作者：
Zhixiong Hu;A. Glidle;C. Ironside;J. Cooper;Huabing Yin
中科院分区：
文献类型：
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作者：
Zhixiong Hu;A. Glidle;C. Ironside;J. Cooper;Huabing Yin
我们描述了一个集成透镜阵列波导光栅（AWG）显微光谱仪的本地化复用荧光测量的发展。该设备的占地面积仅为1 mm宽，1 cm长，能够在芯片上进行光谱测量。基于来自CCD相机的简单强度读数同时测量多个荧光信号。我们还展示了集成的AWG光谱仪与微流体平台，使用透镜功能，以限制光束的形状聚焦照明。这种能力增强了信号收集，提供了更好的空间分辨率，并提供了一种用于分析流动中的小体积样品（例如细胞）的途径。为了展示这些能力，我们开发了一种新的“珠AWG”平台，我们展示了本地化的多重荧光检测同时或相继。这种集成系统为能够从单个限定位置光学检测多波长荧光的便携式系统提供了基础。
We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel "bead-AWG" platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.