Human cathepsin X:: A novel cysteine protease of the papain family with a very short proregion and unique insertions
Human cathepsin X:: A novel cysteine protease of the papain family with a very short proregion and unique insertions
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DOI：
10.1016/s0014-5793(98)00964-8
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发表时间：
1998-08-28
期刊：
影响因子：
3.5
通讯作者：
Ménard, R
中科院分区：
文献类型：
--
作者：
Nägler, DK;Ménard, R
通过 PCR 扩增从人卵巢 cDNA 文库中获得了编码木瓜蛋白酶家族半胱氨酸蛋白酶（称为组织蛋白酶 X）的新型 cDNA。组织蛋白酶 X cDNA 在人体组织中广泛表达，包含 912 个核苷酸的开放阅读框，编码 303 个氨基酸的预测蛋白质。木瓜蛋白酶样半胱氨酸蛋白酶中所有高度保守的区域（包括催化残基）都存在于组织蛋白酶 X 中。组织蛋白酶 X 的成熟部分与人组织蛋白酶 B、C、H、K、L、O、S 和 W 具有 26-32% 的同一性。组织蛋白酶 X 序列包含几个独特的特征：（i）非常短的前区； (ii)在推定的氧阴离子孔的谷氨酰胺和活性位点半胱氨酸之间的高度保守区域中插入三个氨基酸残基； (iii) 第二次插入 15 个氨基酸残基，可与组织蛋白酶 B 中的闭塞环区域对齐。由 Elsevier 代表欧洲生化学会联合会出版。
A novel cDNA encoding a cysteine protease of the papain family named cathepsin X was obtained by PCR amplification from a human ovary cDNA library. The cathepsin X cDNA is ubiquitously expressed in human tissues and contains an open reading frame of 912 nucleotides encoding a predicted protein of 303 amino acids. All highly conserved regions in papain-like cysteine proteases including the catalytic residues are present in cathepsin X. The mature part of cathepsin X is 26-32% identical to human cathepsins B, C, H, K, L, O, S and W, The cathepsin X sequence contains several unique features: (i) a very short proregion; (ii) a three amino acid residue insertion in a highly conserved region between the glutamine of the putative oxyanion hole and the active site cysteine; and (iii) a second insertion of 15 amino acid residues that can be aligned with the occluding loop region in cathepsin B. Published by Elsevier on behalf of the Federation of European Biochemical Societies.