The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens.
The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens.
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DOI：
10.1186/s12864-016-2612-7
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发表时间：
2016-04-18
期刊：
影响因子：
4.4
通讯作者：
Kaiser P
中科院分区：
文献类型：
--
作者：
Psifidi A;Fife M;Howell J;Matika O;van Diemen PM;Kuo R;Smith J;Hocking PM;Salmon N;Jones MA;Hume DA;Banos G;Stevens MP;Kaiser P
弯曲杆菌是人类食源性腹泻病的主要原因，主要是通过食用或处理受污染的禽肉获得的。在缺乏有效的许可疫苗和抑制剂的情况下，选择对弯曲杆菌具有更高抗性的鸡可能会减少其随后进入食物链。弯曲杆菌肠道定殖水平受鸡的宿主遗传学影响。在本研究中，使用两个鸡群体来研究禽类对定殖的抗性的遗传结构：（i）已知对弯曲杆菌定殖的抗性不同的两个白色来航鸡衍生的近交系[（61 x N）x N]的回交和（ii）第9代高级互交（61 x N）系。空肠弯曲菌实验感染后的定殖水平被认为是一个数量性状。使用1，243个完全信息的单核苷酸多态性（SNP）标记的回交实验揭示了染色体7，11和14上的数量性状位点（QTL）。在进一步的互交系研究中，对QTL在第14染色体上的定位进行了确认和精化，并在第4和第16染色体上鉴定出两个新的QTL。通过对QTL候选区域基因的路径和重测序数据分析，确定了潜在的路径、网络和候选抗性基因。最后，对一些候选抗性基因进行基因表达分析以支持结果。鸡的弯曲杆菌抗性是一个复杂的性状，可能涉及主要组织相容性复合体、先天性和适应性免疫应答、钙粘蛋白和其他因素。弯曲杆菌抗性的两个QTL与沙门氏菌抗性基因座共定位，表明有可能同时育种以增强对两种人畜共患病的抗性。本文的在线版本（doi：10.1186/s12864-016-2612-7）包含补充材料，可供授权用户使用。
Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(61 x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9th generation advanced intercross (61 x N) line. The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results. Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses. The online version of this article (doi:10.1186/s12864-016-2612-7) contains supplementary material, which is available to authorized users.