Production of CCL20 on nucleus pulposus cells recruits IL-17-producing cells to degenerated IVD tissues in rat models
Production of CCL20 on nucleus pulposus cells recruits IL-17-producing cells to degenerated IVD tissues in rat models
复制标题
大鼠模型中髓核细胞产生的 CCL20 将产生 IL-17 的细胞募集到退化的 IVD 组织中
DOI：
10.1007/s10735-015-9651-2
复制
发表时间：
2016-02-01
影响因子：
3.2
通讯作者：
Cheng, Lei
中科院分区：
文献类型：
--
作者：
Zhang, Yuanqiang;Liu, Lian;Cheng, Lei
产生IL-17的细胞在各种自身免疫性疾病中发挥重要作用。在椎间盘（IVD）退变患者中观察到更高水平的IL-17产生细胞。然而，IL-17产生细胞的积累机制仍有待阐明。我们的目的是证明CC趋化因子配体（CCL）20和其特异性受体CCR 6的相互作用是否参与了IL-17产生细胞在大鼠模型退变椎间盘组织中的募集。建立了大鼠可接受的针刺模型和自体髓核应用模型。所有动物随机分为假手术组、针刺组（NP组）、假手术+自体髓核应用组（sham-ANPA组）和针刺+自体髓核应用组（NP-ANPA组）。采用免疫组化染色、western blot和实时荧光定量PCR检测IVD标本中CCL 20、IL-17和CCR 6的表达水平。ELISA法检测血清IL-17水平。采用Pearson相关分析CCL 20、CCR 6和IL-17表达之间的相关性。与对照组相比，CCL 20、IL 17和CCR 6的表达显著升高。与假手术组相比，NP-ANPA组血清中的循环IL-17升高。CCL 20、CCR 6和IL-17的表达水平呈正相关。这些结果提示了通过CCL 20/CCR 6系统在体内募集IL-17产生细胞到退变椎间盘的潜在机制。
IL-17-producing cells play an important role in various autoimmune diseases. A higher level of IL-17-producing cells is observed in patients with intervertebral disc (IVD) degeneration. However, the mechanism of the accumulation of IL-17-producing cells remains to be elucidated. Our aim is to demonstrate whether the interaction of CC chemokine ligand (CCL) 20 and its specific receptor CCR6 is involved in the recruitment of IL-17-producing cells to degenerated disc tissues in rat models. The well-accepted needle puncture model and the autologous nucleus pulposus application model of rats were established. All of the animals were randomly divided into four groups: the sham surgery group, the needle puncture group (NP group), the sham surgery + autologous nucleus pulposus application group (sham–ANPA group) and the needle puncture + autologous nucleus pulposus application group (NP–ANPA group). Immunohistochemical staining, western blot and real-time PCR were used to evaluate the expression levels of CCL20, IL-17 and CCR6 in the IVD samples. Moreover, the IL-17 concentrations in the serum were detected by ELISA. Pearson correlations were performed to analyse the correlation among the expressions of CCL20, CCR6 and IL-17. The expressions of CCL20, IL17 and CCR6 were dramatically elevated in comparison with the control groups. The circulating IL-17 in the serum of the NP–ANPA group was elevated compared to the sham surgery group. In addition, there was a positive correlation among the expression levels of CCL20, CCR6 and IL-17. The results suggest a potential mechanism for the recruitment of IL-17-producing cells to degenerated intervertebral discs via a CCL20/CCR6 system in vivo.