The alteration of gene expression in rat liver during chemical carcinogenesis.
The alteration of gene expression in rat liver during chemical carcinogenesis.
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化学致癌过程中大鼠肝脏基因表达的改变。
DOI：
10.1016/0003-9861(83)90528-3
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发表时间：
1983
影响因子：
3.9
通讯作者：
Schwartz,CE
中科院分区：
文献类型：
--
作者：
Chiu,JF;Huang,DP;Burkhardt,AL;Cote,G;Schwartz,CE
本文研究了致癌物3′-甲基-4-二甲氨基偶氮苯（3′-MDAB）对大鼠肝脏基因表达的影响。与丰富的肿瘤肝特异性cDNA的杂交分析表明，在肝癌发生过程中的基因表达的显着变化。以白蛋白和甲胎蛋白（AFP）基因为模型，研究3′-MDAB对肝特异性基因表达的影响。血清AFP水平在肝癌发生过程中显著升高。通过翻译和杂交实验确定多聚体poly（A）mRNA中存在的AFP mRNA的量。随着血清AFP水平的升高，肝癌组AFP mRNA表达也随之升高。肝细胞质RNA中AFPmRNA的表达在给药后18 d即开始增加，在给药后30-40 d和185 d达高峰。血清AFP水平和肝胞液AFP mRNA在两个峰值之间显著下降，在3′-MDAB处理100天的大鼠中可检测到少量AFP mRNA和血清AFP。AFP mRNA量的增加是由于用致癌物处理的大鼠肝脏中AFP基因转录的增加。3′-MDAB处理组大鼠血清白蛋白浓度和肝组织白蛋白mRNA水平无明显变化。
The effect of the carcinogen 3′-methyl-4-dimethylaminoazobenzene (3′-MDAB) on the gene expression of rat liver was studied. Hybridization analysis with enriched neoplastic liver specific cDNA demonstrated a noticeable change in gene expression during liver carcinogenesis. The effect of 3′-MDAB on liver specific gene expression was also studied with albumin and α-fetoprotein (AFP) genes as models. Serum AFP levels increased dramatically during hepatocarcinogenesis. Amounts of AFP mRNA present in polysomal poly(A)mRNA were determined by translation and hybridization experiments. AFP mRNA increased in carcinogen-treated liver as the serum level of AFP increased. Increase of AFP mRNA in liver cytoplasmic RNAs can be detected as early as 18 days after feeding rats 3′-MDAB and reached peaks at 30–40 and 185 days of treatment. AFP serum level and AFP mRNA in liver cytosol decreased dramatically between these peaks, with a small amount of AFP mRNA and serum AFP detectable in rats treated with 3′-MDAB for 100 days. The increase in amount of AFP mRNA was due to an increase in transcription of the AFP gene in livers of rats treated with carcinogen. Only a slight change in serum albumin concentration and albumin mRNA in livers of rats treated with 3′-MDAB was noticed.
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莫里斯肝癌 7777 大鼠中甲胎蛋白和白蛋白的分解代谢。
影响因子：
11.2
作者：
S. Sell
通讯作者：
S. Sell
影响因子：
2.9
作者：
CHAMBERLAIN, JP
通讯作者：
CHAMBERLAIN, JP
正常和 SV40 转化的人成纤维细胞的 mRNA 序列之间存在广泛的同源性
影响因子：
64.5
作者：
R. Hoffman;S. Penman;Jeffrey G. Williams
通讯作者：
Jeffrey G. Williams
喂食 3-甲基-4-二甲基氨基偶氮苯的大鼠肝脏中和由相同致癌物诱导的肝癌中的多聚体聚 (A) RNA。
DOI：
--
发表时间：
1980
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
W. Knöchel;N. Patel;V. Holoubek
通讯作者：
V. Holoubek
影响因子：
5.6
作者：
WETMUR, JG;DAVIDSON, N
通讯作者：
DAVIDSON, N