Identification of proteins interacting with the mitochondrial small heat shock protein Hsp22 of Drosophila melanogaster: Implication in mitochondrial homeostasis.
Identification of proteins interacting with the mitochondrial small heat shock protein Hsp22 of Drosophila melanogaster: Implication in mitochondrial homeostasis.
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DOI：
10.1371/journal.pone.0193771
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Tanguay RM
中科院分区：
文献类型：
--
作者：
Dabbaghizadeh A;Morrow G;Amer YO;Chatelain EH;Pichaud N;Tanguay RM
来自黑腹果蝇（Drosophila melanogaster）的小热休克蛋白（sHsp） Hsp22 （DmHsp22）是该双翅目sHsp家族的一部分。这种sHsp的特征在于它存在于线粒体基质中，并在衰老过程中优先表达。虽然DmHsp22已被证明是一种有效的体外伴侣，但其在体内线粒体中的功能仍不清楚。因此，确定其在线粒体基质中的蛋白质相互作用网络（interactome）将有助于阐明其功能。本研究将免疫亲和偶联法（IAC）与质谱法结合，对转染DmHsp22的HeLa细胞在非热休克和热休克后的线粒体进行了分析。在两个独立的IACs中检测到60个共同的dmhsp22结合线粒体伴侣。免疫印迹法用于验证DmHsp22与线粒体伴侣机制的两个成员之间的相互作用；Hsp60和Hsp70。在DmHsp22的配对体中，发现了几个ATP合酶亚基。此外，我们发现瞬时转染的HeLa细胞中DmHsp22的表达增加了线粒体最大耗氧能力和ATP含量，提供了DmHsp22与线粒体功能之间的机制联系。
The small heat shock protein (sHsp) Hsp22 from Drosophila melanogaster (DmHsp22) is part of the family of sHsps in this diptera. This sHsp is characterized by its presence in the mitochondrial matrix as well as by its preferential expression during ageing. Although DmHsp22 has been demonstrated to be an efficient in vitro chaperone, its function within mitochondria in vivo remains largely unknown. Thus, determining its protein-interaction network (interactome) in the mitochondrial matrix would help to shed light on its function(s). In the present study we combined immunoaffinity conjugation (IAC) with mass spectroscopy analysis of mitochondria from HeLa cells transfected with DmHsp22 in non-heat shock condition and after heat shock (HS). 60 common DmHsp22-binding mitochondrial partners were detected in two independent IACs. Immunoblotting was used to validate interaction between DmHsp22 and two members of the mitochondrial chaperone machinery; Hsp60 and Hsp70. Among the partners of DmHsp22, several ATP synthase subunits were found. Moreover, we showed that expression of DmHsp22 in transiently transfected HeLa cells increased maximal mitochondrial oxygen consumption capacity and ATP contents, providing a mechanistic link between DmHsp22 and mitochondrial functions.
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DOI：
10.1186/2046-2395-3-6
发表时间：
2014
期刊：
Longevity & healthspan
影响因子：
--
作者：
Dai DF;Chiao YA;Marcinek DJ;Szeto HH;Rabinovitch PS
通讯作者：
Rabinovitch PS
影响因子：
12.3
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DOI：
10.1073/pnas.152330499
发表时间：
2002-12-10
影响因子：
11.1
作者：
Bonini, NM
通讯作者：
Bonini, NM
影响因子：
11.8
作者：
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通讯作者：
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影响因子：
7
作者：
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