A novel strategy using single-chain antibody to show the importance of Bcl-2 in mast cell survival
A novel strategy using single-chain antibody to show the importance of Bcl-2 in mast cell survival
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DOI：
10.1182/blood-2002-12-3921
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发表时间：
2003-10-01
期刊：
影响因子：
20.3
通讯作者：
Razin, E
中科院分区：
文献类型：
--
作者：
Cohen-Saidon, C;Nechushtan, H;Razin, E
细胞凋亡或程序性细胞死亡在多种生理过程中发挥重要作用，受Bcl-2家族蛋白的调控，该家族蛋白包括抗凋亡因子和促凋亡因子。Bcl-2蛋白家族在肥大细胞凋亡过程中的直接参与尚不清楚。在目前的工作中，我们使用了从半合成的人噬菌体展示抗体库中提取的针对Bcl-2的单链抗体（scFv）。TAT序列的加入，负责通过细胞膜进行易位，赋予抗bcl -2- scfv穿透活细胞的能力。此外，它通过结合BH1结构域在细胞内特异性中和Bcl-2，并在2种肥大细胞和人乳腺癌细胞系中消除其抗凋亡活性。(C) 2003年由美国血液病学会出版。
Apoptosis or programmed cell death plays an important role in a wide variety of physiologic processes and is regulated by proteins of the Bcl-2 family consisting of both antiapoptotic and proapoptotic factors. The direct involvement of the Bcl-2 protein family in the process of mast cell apoptosis has not been clarified. In the present work we have used a single-chain antibody (scFv) raised against Bcl-2 derived from a semisynthetic human phage-display antibody library. The addition of TAT sequence, which is responsible for translocation through the membrane, endows the antiBcl-2-scFv with the ability to penetrate living cells. Moreover, it specifically neutralizes Bcl-2 intracellularly by binding to the BH1 domain and eradicates its antiapoptotic activity in 2 types of mast cells and in a human breast cancer cell line. (C) 2003 by The American Society of Hematology.